The publication of Claes
The function of this ligament is to provide anterolateral knee stability by preventing the lateral tibia from subluxation anteriorly relative to the femur(9,10). Studies have documented ALL injuries in 46% to 79% in combination with ACL injuries(11,12). Persisting rotational instability after ACL reconstruction (prevalence 25%)(9) seems to be due to insufficiency of these lateral structures(13). Combined reconstruction of the ALL and ACL should be considered depending on patient history, clinical signs, imaging, and patient profile(9,14).
Musculoskeletal ultrasonography (US) is a non-invasive, cost-effective, and valid method to visualize extraarticular structures in real time. Only a few studies investigated the visualization of the ALL with US(15–19). These studies were unable to determine how to reliably visualize the ALL with US(20). It is hypothesized that point-of-care ultrasound (POCUS) of the ALL may be useful if an ACL tear is suspected. However, a standardized protocol in how to reliably visualize the ALL with US is needed.
The purpose of this study is to present a standardized protocol to visualize the ALL with US and to determine interrater reliability.
Neri
To consider a target population at risk, healthy active people between 18 and 55 years were included, by non-probability sampling. Informed consent was obtained from each participant, and the rights of human subjects were protected. Three raters, all physical therapists, MSc, with at least 3 years of experience in US (MAP, ML, MK) performed all measurements. Each sonographer completed a 4 hour training session in order to get familiar with the ALL protocol prior to this study. The volunteer underwent a clinical examination by an experienced physical therapist to exclude clinical anterolateral instability.
To visualize the ALL in vivo a standardized protocol based on the validation of the scanning position was defined with the participant lying on the ipsilateral side with the upper leg in 30° flexion and his foot hanging off the examination table. A pillow was placed under his knee. The examiner sat behind the participant in order to easily adjust US settings and be able to flex and rotate the leg, if necessary. The ITB was located in its long axis at its insertion on Gerdy’s tubercle. Then, the probe was slowly rotated towards the fibular head. Halfway between Gerdy’s tubercle and head of the fibula, the distal insertion of the ALL can be found. In this position, the popliteus tendon and the lateral inferior genicular artery (LIGA) were used as landmarks to identify the ALL running to the lateral femur condyle. In this position (Fig. 4), length, thickness (just above the LIGA) and distance from insertion to tibia plateau were measured. The width of the ALL was measured in a short axis view above the LIGA. Images and measurements were recorded and stored on the US system.
For the three raters (
The three raters performed measurements of the ALL with a Philips Affiniti 50G (Royal Philips, Amsterdam, The Netherlands) ultrasound device and an 18-5 MHz, linear 5 cm transducer on both legs of the participant.
Data analyses were performed using IBM SPSS Version 21 (SPSS Inc. Chicago, Il, USA). All data was checked for assumptions. Descriptive statistics were calculated, and the Intraclass Correlation Coefficient (ICC) was used to assess inter-rater reliability (ICC2.1; two-way random effects model, single measurement type defined in absolute agreement) reported with 95% confidence interval (CI) of the estimated ICC. The level of reliability was based on the general guideline according to Koo, 2016(25).
In the Thiel embalmed specimen the needle markings placed with US corresponded exactly to the ALL position observed at subsequent dissection and confirmed by an experienced anatomist (EC, 20 years of experience). Eighteen healthy subjects (12 male and 6 female) participated in this study. Mean (± standard deviation) of age, height, and weight were 41.2 (±10.3) years, 179.2 (±8.5) cm and 84.7 (±13.1) kg. From 36 knees, two were excluded due to prior surgery. There were no signs of clinical anterolateral instability in the remaining 34 knees. The three raters were able to localize the ALL in 33 knees (97%). Characteristics of the participants and ALL are shown in Table 1. The ICC was calculated as the data was normally distributed. The inter-rater reliability of ALL thickness was poor, ICC = 0.35 (95% CI: -0.6-0.63). The inter-rater reliability was good for of ALL length and width, ICC 0.80 (95% CI: 0.64-0.89) and 0.88 (95% CI: 0.79–0.94), respectively; and excellent for the distance between insertion of the ALL and lateral tibia plateau, ICC 0.96 (95% CI: 0.93-0.98), as seen in Tab. 2.
|
Lower | Higher | Mean | SD | |
---|---|---|---|---|---|
Age (year) | 33 | 23 | 54 | 41.2 | 10.3 |
Height (cm) | 33 | 164 | 188 | 179.2 | 8.5 |
Weight (kg) | 33 | 63.3 | 104 | 84.7 | 13.1 |
BMI | 33 | 21.5 | 31.1 | 26.3 | 2.9 |
ALL length | 33 | 38.7 | 53.3 | 46.9 | 4.2 |
ALL thickness | 33 | 0.52 | 1.24 | 0.94 | 0.16 |
ALL width | 33 | 4.4 | 11.7 | 8.4 | 2.3 |
Distance to tibia plateau | 33 | 2.2 | 9.2 | 5.7 | 1.8 |
Intraclass correlation | 95% confidence interval | Significance | ||
---|---|---|---|---|
Lower bound | Upper bound | |||
ALL length | 0.799 | 0.643 | 0.894 | <0.001 |
ALL thickness | 0.346 | -0.058 | 0.0632 | 0.038 |
ALL width | 0.884 | 0.794 | 0.939 | <0.001 |
Distance to tibia plateau | 0.959 | 0.927 | 0.978 | <0.001 |
US has been used to visualize the ALL in five previous studies. Cianca
After evaluating US in a cadaveric observation, we determined the inter-rater reliability in 34 healthy knees. Mean length and width of the ALL in our study was 46.9 (±4.2) mm, resp. 8.4 (±2.3) mm, which corresponded well to with Neri
In our study the Intraclass Correlation Coefficient (ICC) was poor for ALL thickness, good for ALL length and width and excellent for the distance between insertion and lateral tibia plateau. Our findings are consistent with prior studies(3,17), that reported variability in the femoral attachment and a strong connection between the structures around the lateral femoral condyle, making the ALL more difficult to identify in this area. The poor consistency in the ALL thickness measurement may be due to the accuracy of US measuring beyond millimetres. A small measurement error of 0.1 mm, indicates a difference of 11% to the mean thickness (0.9 mm). Faruch
Nearly perfect reliability of the measurement of the distal insertion of the ALL has important implications for patient care. Almost all injuries of the ALL are located in the distal insertion of the ligament. Faruch
Our study has several limitations. Healthy active participants were included in our study. Our results may not hold up in patients suspected of having an ACL tear, where there might be pathological changes of the ALL. Integrity instead of morphologic characteristics should be evaluated in this situation. Studies in pathological cases are needed. Validation of the ultrasound protocol was done on a single Thiel embalmed cadaveric knee. The advantage of this embalming method is that the specimens retain tissue characteristic as in a fresh specimen in contradistinction to classic embalming where tissues harden and tissue planes become inseparable. Despite a good agreement between US and dissection, this can only be considered as a minimal validation. The mean age of the participants (41 years) did not entirely match the mean age of people at risk for an ACL tear (18 to 25 years)(28).
The US protocol presented is reliable for evaluating the anterolateral ligament of the knee. There is an excellent reliability for the distal part of the ALL. As injuries typically occur in this part of the ligament, our protocol is promising for evaluating the ALL in patients with suspected ACL tears in clinical practice and on the playfield.