1. bookVolume 66 (2017): Issue 1 (December 2017)
Journal Details
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Format
Journal
eISSN
2509-8934
First Published
22 Feb 2016
Publication timeframe
1 time per year
Languages
English
Open Access

Isolation and characterization of microsatellite markers from the RAD sequence of two temperate liana species: Euonymus fortunei (Celastraceae) and Schizophragma hydrangeoides (Hydrangeaceae)

Published Online: 28 Dec 2017
Volume & Issue: Volume 66 (2017) - Issue 1 (December 2017)
Page range: 40 - 42
Journal Details
License
Format
Journal
eISSN
2509-8934
First Published
22 Feb 2016
Publication timeframe
1 time per year
Languages
English
Abstract

Clonal reproduction of lianas is a common but important life history strategy. It is necessary to evaluate the clonal structure of liana species because clonal ability is potentially a major determinants distribution pattern of lianas. Therefore, we developed 10 microsatellite markers for Euonymus fortunei and Schizophragma hydrangeoides respectively from genomic sequences obtained from double-digest restriction site associated DNA (ddRAD). The sequence data of the developed markers were deposited on the public database. The expected heterozygosity (HE) of E. fortunei and S. hydrangeoides ranged from 0.727 to 0.847 with an average of 0.766, and from 0.734 to 0.924 with an average of 0.812, respectively. All loci were under HWE except for a locus of S. hydrangeoides (sh07). These markers should contribute to the understanding of the life history of temperate liana species.

Keywords

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