1. bookVolume 63 (2014): Issue 1-6 (December 2014)
Journal Details
License
Format
Journal
eISSN
2509-8934
First Published
22 Feb 2016
Publication timeframe
1 time per year
Languages
English
access type Open Access

Breeding triploid aspen and poplar clones for biomass production

Published Online: 01 Jun 2017
Volume & Issue: Volume 63 (2014) - Issue 1-6 (December 2014)
Page range: 47 - 58
Received: 25 Jun 2013
Journal Details
License
Format
Journal
eISSN
2509-8934
First Published
22 Feb 2016
Publication timeframe
1 time per year
Languages
English
Abstract

Enriched diploid pollen was applied for in vitro pollinations and crossbreeding in the greenhouse to produce high performance triploid aspen and aspen hybrids for cultivation in medium rotation plantations. In addition to crossings within the section Populus, intersectional crossbreeding was performed to combine benefits of intersectional hybridization with those derived from triploidisation.

Both the enrichment of diploid pollen by size fractionation of naturally unreduced pollen and heat treatment of microspore mother cells resulted in a distinct increase of diploid pollen. Using this pollen, six triploid plants were obtained from in vitro pollinations and twenty from crossbreeding in the greenhouse. The triploid plants displayed a high variability in growth performance. Two clones from in vitro pollination and five from crossbreeding in the greenhouse were chosen to estimate growth characteristics. A first assessment of clone performance in an outdoor container test con - ducted over one growing season revealed two triploid clones with a same stem height and a significantly increased basal stem diameter in comparison to the fast-growing triploid reference clone “Astria”. Crossbreeding experiments also resulted in two fast-growing mixoploid clones, which have already been stable for several years.

All in all, in this study, crossbreeding using enriched diploid pollen is proved to be a reliable and applicable approach for an effective breeding of triploid poplars.

Keywords

ADLER, P. R., S. J. DEL GROSSO and W. J. PARTON (2007): Life-cycle assessment of net greenhouse-gas flux for bioenergy cropping systems. Ecol Applic 17: 675-691.10.1890/05-201817494388Search in Google Scholar

BAUMEISTER, G. (1980): Beispiele der Polyploidie-Züchtung. AFZ 26: 340-343.Search in Google Scholar

BRADSHAW, H. D. and R. F. STETTLER (1993): Molecular genetics of growth and development in Populus. I. Triploidy in hybrid poplars. Theor Appl Genet 86: 301-307.10.1007/BF0022209224193473Search in Google Scholar

BRETAGNOLLE, F. and J. D. THOMPSON (1995): Gametes with the somatic chromosome number: mechanisms of their formation and role in the evolution of autoploid plants. New Phytol 129: 1-22.10.1111/j.1469-8137.1995.tb03005.x33874422Search in Google Scholar

CAI, X. and X.Y. KANG (2011): In vitro tetraploid induction from leaf explants of Populus pseudo-simonii Kitag. Plant Cell Rep 30: 1771-1778.10.1007/s00299-011-1085-z21750904Search in Google Scholar

DE LA TORRE UGARTE, D. G., M. E. WALSH, H. SHAPOURI and S. P. SLINSKY (2003): The economic impacts of bioenergy crop production on U.S. agriculture. U.S.D.A. Office of Chief Economist, Office of Energy Policy and New Uses. Rpt. No. 816, 41p.Search in Google Scholar

DE STORME, N. and D. GEELEN (2011): The Arabidopsis mutant Jason produces unreduced First Division Restitution male gametes through a parallel/fused spindle mechanism in Meiosis II. Plant Physiol 155: 1403-1415.10.1104/pp.110.170415304659421257792Search in Google Scholar

DEWITTE, A., K. VAN LAERE and J. VAN HUYLENBROECK (2012): Use of 2n Gametes in Plant Breeding. pp. 59-86. In: Plant Breeding, edited by I.Y. ABDURAKHMONOV, www.intechopen.com.10.5772/29827Search in Google Scholar

ECKENWALDER, J. E. (1996): Systematics and evolution of Populus. pp. 7-32. In: Biology of Populus and its implications for management and conservation, edited by R. F. STETTLER, H. D. BRADSHAW JR., P. E. HEILMANN and T. M. HINCKLEY, NRC Research Press, National Research Council of Canada, Ottawa.Search in Google Scholar

EINSPAHR, D.W., J. P. VAN BUIJTENEN and J. R. PECKHAM (1963): Natural variation and heritability in triploid aspen. Silvae Genet. 12: 51-58.Search in Google Scholar

EINSPAHR, D.W. and L. L. WINTON (1976): Genetics of quaking aspen. Aspen Bibliography, Paper 5026. http://digitalcommons.Usu.edu/aspen_bib/5026.Search in Google Scholar

EINSPAHR, D.W. (1984): Production and utilisation of triploid hybrid aspen. Iowa State J. Res. 58: 401-409.Search in Google Scholar

EVERY, D. A. and D. WIENS (1971): Triploidy in Utah aspen. Madrono: 138-47.Search in Google Scholar

EWALD, D., K. ULRICH, G. NAUJOKS and M. B. SCHRÖDER (2009): Induction of tetraploid poplar and black locust plants using colchicine: chloroplast number as an early marker for selecting polyploids in vitro. Plant Cell Tiss Organ Cult 99: 353-357.10.1007/s11240-009-9601-3Search in Google Scholar

EWALD, D. and K. ULRICH (2012): In vitro pollination in poplar of section Populus. Plant Cell Tiss Organ Cult 111: 255-258.10.1007/s11240-012-0189-7Search in Google Scholar

HARLAN, J. R. and J. M. J. DE WET (1975): On Ö. Winge and a prayer: The origins of polyploidy. Bot Rev 41: 361-390.10.1007/BF02860830Search in Google Scholar

HARTMANN, H. T., D. E. KESTER, F. T. DAVIES JR. and R. L. GENEVE (2002): Hartmann and Kester’s plant propagation: principles and practices. 7th ed. Pearson education, New Jersey. ISBN 0-13-679235-9.Search in Google Scholar

HEILBORN, O. (1934): On the origins and preservation of polyploidy. Hereditas 19: 233-242.10.1111/j.1601-5223.1934.tb02624.xSearch in Google Scholar

HERRMANN, S. (1976): Verfahren zur Konservierung und Erhaltung der Befruchtungsfähigkeit von Waldbaumpollen über mehrere Jahre. Silvae Genet 25: 223-229.Search in Google Scholar

JANSSEN, A., S. FEHRENZ, C. FEY-WAGNER and W. HÜLLER (2012): Züchtung von Schwarz- und Balsampappeln für en Kurzumtrieb, pp. 33-54. In: Züchtung und Ertragsleistung schnell wachsender Baumarten im Kurzumtrieb. Beiträge aus der Nordwestdeutschen Forstlichen Versuchsanstalt, Universitätsverlag Göttingen.Search in Google Scholar

JOHNSSON, H. and C. EKLUNDH (1940): Colchicine treatment as a method in breeding hardwood species. Svensk Papp Tidn 43: 373-377.Search in Google Scholar

KANG, X.Y. and Z. T. ZHU (1997): A study on the 2n pollen vitality and germinant characteristics of white populus. Acta Bot Yunnanica 19: 402-406.Search in Google Scholar

KANG, X.Y., Z. T. ZHU and H. B. LIN (1999): Study on the effective treating period for pollen chromosome doubling of P. tomentosa x P. bolleana. Sci Silvae Sin 35: 21-24.Search in Google Scholar

KANG, X.Y., Z. T. ZHU and H. B. LIN (2000): Radiosensitivity of different ploidy pollen in poplar and its application. Acta Genet Sinica 27: 78-82.Search in Google Scholar

KANG, X.Y., Z. T. ZHU and Z.Y. ZHANG (2000a): Suitable period of high temperature treatment for 2n pollen of Populus tomentosa x P. bolleana. J Beijing For Univ 22: 1-4.Search in Google Scholar

KANG, X.Y. (2002): Mechanism of 2n pollen occurring in Chinese white poplar. J Beijing For Univ 24: 67-70.Search in Google Scholar

KANG, X.Y., J. ZHANG, P. D. ZHANG and Y. H. LI (2006): Induction of triploids in white poplar, pp. 71. In: International Poplar Symposium IV, Nanjing.Search in Google Scholar

KUNAKH, V. A., V. I. ADONIN, S. P. OZHEREDOV and Y. B. BLYUM (2008): Mixoploidy in wild and cultivated species of Cruciferae capable of hybridizing with rapeseed Brassica napus. Cytol Genet 42: 204-209.10.3103/S0095452708030079Search in Google Scholar

LI, Y. H., X.Y. KANG, S. D. WANG, Z. H. ZHANG and H.W. CHEN (2008): Triploid induction in Populus alba x P. glandulosa by chromosome doubling of female gametes. Silvae Genet 57: 37-40.10.1515/sg-2008-0006Search in Google Scholar

LIESEBACH, H., G. NAUJOKS and D. EWALD (2011): Successful hybridisation of hybrid aspen (Populus tremula x P. tremuloides) and eastern cottonwood (P. deltoides). Sex Plant Reprod 24: 189-198.10.1007/s00497-010-0156-621107610Search in Google Scholar

LINSMAIER, E. M. and F. SKOOG (1965) Organic growth factor requirements of tobacco tissue culture. Plant Physiol 21: 487-492.10.1111/j.1399-3054.1965.tb06874.xSearch in Google Scholar

LIU, G., Z. LI and M. BAO (2007): Colchicine-induced chromosome doubling in Platanus acerifolia and its effect on plant morphology. Euphytica 157: 145-154.10.1007/s10681-007-9406-6Search in Google Scholar

MASHKINA, O. S., I. M. BURDAEVA, M. M. BELOZEROVA and L. N. V’YUNOVA (1989) A method of inducing diploid pollen in woody species. Lesovedenie 1: 19-25.Search in Google Scholar

MOCK, K. E., C. M. CALLAHAN, M. N. ISLAM-FARIDI, J. D. SHAW, H. S. RAI and S. C. SANDERSON et al. (2012): Widespread Triploidy in Western American Aspen (P. tremuloides). PLoS ONE 7 (10): e48406.Search in Google Scholar

MÜNTZING, A. (1936): The chromosomes of a giant Populus tremula. Hereditas 21: 383-393.10.1111/j.1601-5223.1936.tb03206.xSearch in Google Scholar

NILSSON-EHLE, H. (1936): Note regarding the gigas form of Populus tremula found in nature. Hereditas 21: 372-82.Search in Google Scholar

ORJEDA, G., R. FREYRE and M. IWANAGA (1990): Production of 2n pollen in diploid Ipomoea trifida, a putative wild ancestor of sweet potato. J Hered 81: 462-467.10.1093/oxfordjournals.jhered.a111026Search in Google Scholar

OKORO, O. O. and GRACE, J. (1976): The physiology of rooting Populus cuttings. I. Carbohydrates and Photosynthesis. Physiol Plant 36: 133-138.10.1111/j.1399-3054.1976.tb03923.xSearch in Google Scholar

ORTIZ, R. (1997): Occurrence and inheritance of 2n pollen in Musa. Ann Bot 79: 449-453.10.1006/anbo.1996.0367Search in Google Scholar

PAGLIARINI, M. S. (2000): Meiotic behaviour of economically important plant species: the relationship between fertility and male sterility. Genet Mol Biol 23: 997-1002.10.1590/S1415-47572000000400045Search in Google Scholar

PERLACK, R. D., L. L. WRIGHT, A. F. TURHOLLOW, R. L. GRAHAM, B. J. STOKES and D. C. ERBACH (2005): Biomass and feedstockfor a bioenergy and Bioproducts industry: The technical feasibility of a billion-ton annual supply. Oak Ridge National Laboratory, Oak Ridge, TN.10.2172/1216415Search in Google Scholar

RANJBAR, M., R. KARAMIAN and S. NOURI (2011): Diploidtetraploid mixoploidy in a new species of Astragalus (Fabaceae) from Iran. Ann Bot Fennici 48: 343-351.10.5735/085.048.0406Search in Google Scholar

R CORE TEAM (2013): R: A language and environment for statistical computing. R Foundation for Statistical Computing, Vienna, Austria. ISBN 3-900051-07-0, Available at: http://www.R-project.org/.Search in Google Scholar

RIEMENSCHNEIDER, D. E., W. E. BERGUSON, D. I. DICKMANN, R. B. HALL, J. G. ISEBRANDS, C. A. MOHN, G. R. STANOSZ and G. A. TUSKAN (2001): Poplar breeding and testing strategies in the north-central ANDS.: Demonstration of potential yield and consideration of future research needs. The Forestry Chronicle 77: 245-253.10.5558/tfc77245-2Search in Google Scholar

RONALD, W. G. (1982): Intersectional hybridization of Populus sections, Leuce-Aigeiros and Leuce-Tacamahaca. Silvae Genet 31: 94-99.Search in Google Scholar

SAX, K. (1936): The experimental production of polyploidy. J Arnold Arbor 17: 153-59.10.5962/p.185351Search in Google Scholar

SCHENK, R. U. and A. C. HILDEBRANDT (1972): Medium and techniques for induction and growth of monocolyledonous and dicotyledonous plant cell cultures. Can J Bot 50: 199-204.10.1139/b72-026Search in Google Scholar

SCHLARBAUM, S. E. (1991): Cytogenetics of forest treespecies, pp. 593-618. In: Chromosome engineering in plant genetics and breeding, edited by T. TSUCHIYA and P. K. GUPTA, Elsevier Publ., Netherlands.10.1016/B978-0-444-88260-8.50037-9Search in Google Scholar

SIVOLAPOV, A. and T. A. BLAGODAROVA (1994): Different levels of mixoploidy in hybrid poplars, pp. 311-316. In: Cytogenetic studies of forest trees and shrub species, edited by Z. BORZAN and S. E. SCHLARBAUM, Proceedings of the first IUFRO Cytogenetics Working Party, September 8-11, 1993 Brijuni National Park Croatia.Search in Google Scholar

SKIEBE, K. (1965): Die Entstehung von polyploiden Populationen in der Evolution. Deutsch Landw (Berlin) 16: 340-343Search in Google Scholar

STANTON, B., J. EATON, J. JOHNSON, D. RICE, B. SCHUETTE and B. MOSER (2002): Hybrid poplar in the Pacific Northwest: The effects of market-driven management. J For 100: 28-33.Search in Google Scholar

STANTON, B. J., D. B. NEALE and S. LI (2010): Populus Breeding: From the classical to the genetic approach, pp. 309-312. In: Genetics and Genomics of Populus, Plant Genetics and Genomics: Crops and Models 8, edited by S. JANSSON et al. Springer Science+Business Media, DOI 10.1007/978-1-4419-1541-2_14.Search in Google Scholar

TANG, Z. Q., D. L. CHEN, Z. J. SONG, Y. C. HE and D. T. CAI (2010): In vitro induction and identification of tetraploid plants of Paulownia tomentosa. Plant Cell Tiss Organ Cult 102: 213-20.10.1007/s11240-010-9724-6Search in Google Scholar

VÄINÖLÄ, A. (2000): Polyploidization and early screening of Rhododendron hybrids. Euphytica 112: 239-44.10.1023/A:1003994800440Search in Google Scholar

VAN BUIJTENEN, J. P., P. N. JORANSON and D.W. EINSPAHR (1957): Naturally occurring triploid quaking aspen in the United States. Proc Soc Am Foresters: 62-64.Search in Google Scholar

VAN BUIJTENEN, J. P., P. N. JORANSON and D.W. EINSPAHR (1958): Diploid versus triploid aspen as pulpwood sources with reference to growth, chemical, physical, and pulping differences. TAPPI J 41: 170-175.Search in Google Scholar

VEILLEUX, R., N. A. MCHALE and F. I. LAUER (1982): 2n gametes in diploid Solanum: frequency and type of spindle abnormalities. Can J Genet Cytol 24: 301-314.10.1139/g82-032Search in Google Scholar

WANG, J., X. KANG, D. Li, H. CHEN and P. ZHANG (2010): Induction of diploid eggs with colchicine during embryo sac development in Populus. Silvae Genet 59: 40-48.10.1515/sg-2010-0005Search in Google Scholar

WANG, J., D. L. LI and X.Y. KANG (2012): Induction of unreduced megaspores with high temperature during megasporogenesis in Populus. Ann For Sci 69: 59-67.10.1007/s13595-011-0152-5Search in Google Scholar

WRIGHT L. L. (1994): Production technology status of woody and herbaceous crops. Biomass and Bioenergy 6: 191-20910.1016/0961-9534(94)90075-2Search in Google Scholar

XI, X. J., X. B. JIANG, D. LI, L. Q. GUO, J. F. ZHANG, Z. Z. WEI and B.L. LI (2011): Induction of 2n pollen by colchicine in Populus x popularis and its triploids breeding. Silvae Genet 60: 155-60.10.1515/sg-2011-0021Search in Google Scholar

YAO, C. L. and J.W. PU (1998): Timber characteristics and pulp properties of the triploid of Populus tomentosa. J Beijing For Univ 20: 18-21.Search in Google Scholar

ZHANG, Z.Y. and F. L. LI (1992): The techniques of pollen chromosome doubling of Populus tomentosa. J Beijing For Univ 14: 52-58.Search in Google Scholar

ZHANG, Z. and X. KANG (2010): Cytological characteristics of numerically unreduced pollen production in Populus tomentosa-Carr. Euphytica 173: 151-59.10.1007/s10681-009-0051-0Search in Google Scholar

ZHU, Z., X. KANG and Z. ZHANG (1997): Advances in the triploid breeding program of Populus tomentosa in China. J Beijing For Univ 6: 1-8.Search in Google Scholar

ZHU, Z., X.Y. KANG and Z. ZHANG (1998): Studies on selection of natural triploids of Populus tomentosa. Sci Silvae Sin 34: 22-31.Search in Google Scholar

Recommended articles from Trend MD

Plan your remote conference with Sciendo