1. bookVolume 61 (2012): Issue 1-6 (December 2012)
Journal Details
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Format
Journal
eISSN
2509-8934
First Published
22 Feb 2016
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1 time per year
Languages
English
access type Open Access

Cross-Species Amplification of Microsatellite Loci for the Endangered Conifer, Taxus chinensis var. mairei (Taxaceae)

Published Online: 01 Aug 2017
Page range: 287 - 291
Received: 20 Jun 2012
Journal Details
License
Format
Journal
eISSN
2509-8934
First Published
22 Feb 2016
Publication timeframe
1 time per year
Languages
English
Abstract

Being an economical and endangered species, microsatellite markers of Taxus chinensis var. mairei were very limited. We have developed a set of microsatellite markers, which was benefit for future genetic analysis of this rare species. Polymorphic loci were developed from congeneric species by cross-species amplification methods, and new primers were redesigned to test for potential null alleles. 15 loci showed polymorphism. The number of alleles per locus varied from 2 to 23 tested in 48 individuals. The observed heterozygosity (Ho) and expected heterozygosity (He) values ranged form 0.000 to 0.854 and 0.082 to 0.827, respectively. Newly redesigned primer confirmed that no null allele existed in most suspected loci. These microsatellite markers will be useful for future genetic analysis and conservation of this endangered species.

Keywords

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