1. bookVolume 61 (2012): Issue 1-6 (December 2012)
Journal Details
License
Format
Journal
eISSN
2509-8934
First Published
22 Feb 2016
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1 time per year
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English
access type Open Access

Microarray Analysis of Gene Expression in Triploid Black Poplar

Published Online: 01 Aug 2017
Volume & Issue: Volume 61 (2012) - Issue 1-6 (December 2012)
Page range: 148 - 157
Received: 19 Nov 2011
Journal Details
License
Format
Journal
eISSN
2509-8934
First Published
22 Feb 2016
Publication timeframe
1 time per year
Languages
English
Abstract

Triploidy is a widespread phenomenon in cultivated and natural breeding plants and it can confer some growth advantages. Here, we analyzed genome-wide gene expression in triploid Populus euramericana (black poplar) using the Affymetrix poplar microarray to detect any possible correlation between triploid vigor and a unique gene expression profile. Among the 38,400 transcripts that were detected in triploid poplar, 1,564 and 2,015 genes were up- or downregulated, respectively, compared with the diploid. The majority of the upregulated genes in the triploid were associated with carbon and nitrogen metabolism, especially lignin and secondary metabolism. Other genes upregulated in the triploid included genes involved in sugar transport, and brassinosteroid (BR) and auxin metabolism. Downregulated genes were mostly related to the assembly and biosynthesis of ribosomes and the nucleosome macromolecular complex. The results suggested that BR and auxin levels were crucial in controlling sugar transport, photosynthesis and cell wall biosynthesis. Downregulated genes were associated with chromatin regulation in the triploid. The information from this analysis could provide an insight into the vigor of triploid poplar.

Keywords

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