<abstract xmlns="http://www.w3.org/1999/xhtml"><italic>Anadenanthera colubrina</italic> var <italic>cebil</italic> is a tree species native to the Upper Parana Atlantic Forest where human activities have severely impacted causing deep fragmentation. Microsatellites are not available in this species. Therefore, the first objective of this study was to generate chloroplast simple sequence repeats (cpSSR) by cross-species transfer. Understanding the evolutionary dynamics of subdivided populations is an important matter. In this way, a first approach to the characterization of the haplotypic diversity within and between populations as well as the genetic structure of native Argentinean populations were the main goals of this study.Twenty four individuals from two populations of the Misiones province were studied and four cpSSR <italic>loci</italic> were tested. Two of them exhibited polymorphic patterns leading to the identification of 11 cpDNA haplotypes with high mean genetic diversity (<italic>GD</italic>=0.73). The minimum spanning network defined three clear groups which can be assigned to at least three subpopulations. AMOVA indicated that the total variance showed the highest percentage of variation (48%) within subpopulations with a fixation index (FST) statistically significant (FST=0.520; p&lt;0.05). Brown’s two <italic>loci</italic> component analysis indicated that substructure population is present. Jost’s differentiation global index (Dest) was 0.049 while Dest pairwise comparison reflected a certain level of genetic structure.The high diversity level detected in the adult trees of <italic>A. colubrina</italic> var <italic>cebil</italic> from the populations under study could be due to recent human influence. In this way, the reduction in population size caused a reduction in the number of trees leading to surviving trees showing the historical diversity of the populations analyzed.</abstract>

Anadenanthera colubrina var cebil is a tree species native to the Upper Parana Atlantic Forest where human activities have severely impacted causing deep fragmentation. Microsatellites are not available in this species. Therefore, the first objective of this study was to generate chloroplast simple sequence repeats (cpSSR) by cross-species transfer. Understanding the evolutionary dynamics of subdivided populations is an important matter. In this way, a first approach to the characterization of the haplotypic diversity within and between populations as well as the genetic structure of native Argentinean populations were the main goals of this study.Twenty four individuals from two populations of the Misiones province were studied and four cpSSR loci were tested. Two of them exhibited polymorphic patterns leading to the identification of 11 cpDNA haplotypes with high mean genetic diversity (GD=0.73). The minimum spanning network defined three clear groups which can be assigned to at least three subpopulations. AMOVA indicated that the total variance showed the highest percentage of variation (48%) within subpopulations with a fixation index (FST) statistically significant (FST=0.520; p<0.05). Brown’s two loci component analysis indicated that substructure population is present. Jost’s differentiation global index (Dest) was 0.049 while Dest pairwise comparison reflected a certain level of genetic structure.The high diversity level detected in the adult trees of A. colubrina var cebil from the populations under study could be due to recent human influence. In this way, the reduction in population size caused a reduction in the number of trees leading to surviving trees showing the historical diversity of the populations analyzed.

Genetic diversity of <italic>Anadenanthera colubrina</italic> Vell. (Brenan) var <italic>cebil</italic>, a tree species from the South American subtropical forest as revealed by cpSSR markers

Departamento de Genética. Facultad de Ciencias Exactas, Químicas y Naturales

Centro de Estudios Fotosintéticos y Bioquímicos

Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET)

Silvae Genetica

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Genetic diversity of  Vell. (Brenan) var , a tree species from the South American subtropical forest as revealed by cpSSR markers

Anadenanthera colubrina var cebil is a tree species native to the Upper Parana Atlantic Forest where human activities have severely impacted causing deep...

Genetic diversity of Anadenanthera colubrina Vell. (Brenan) var cebil, a tree species from the South American subtropical forest as revealed by cpSSR markers

Published Online: Aug 05, 2017

Page range: 123 - 132

Received: Apr 14, 2010

DOI: https://doi.org/10.1515/sg-2011-0017

Keywords
Upper Parana Atlantic Forest, Curupay, intraspecific genetic diversity, cpSSR cross-species transfer

© 2011 M. E. Barrandeguy et al., published by De Gruyter Open

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Genetic diversity of Anadenanthera colubrina Vell. (Brenan) var cebil, a tree species from the South American subtropical forest as revealed by cpSSR markers

Published Online: Aug 05, 2017

Page range: 123 - 132

Received: Apr 14, 2010

DOI: https://doi.org/10.1515/sg-2011-0017

KeywordsUpper Parana Atlantic Forest, Curupay, intraspecific genetic diversity, cpSSR cross-species transfer

© 2011 M. E. Barrandeguy et al., published by De Gruyter Open

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Keywords
Upper Parana Atlantic Forest, Curupay, intraspecific genetic diversity, cpSSR cross-species transfer