1. bookVolume 58 (2009): Issue 1-6 (December 2009)
Journal Details
License
Format
Journal
eISSN
2509-8934
First Published
22 Feb 2016
Publication timeframe
1 time per year
Languages
English
access type Open Access

Identification and Characterization of Microsatellite Markers Useful for Genetic Analysis of Black Spruce (Picea mariana (Mill.) Populations

Published Online: 19 Oct 2017
Volume & Issue: Volume 58 (2009) - Issue 1-6 (December 2009)
Page range: 168 - 172
Received: 28 Apr 2008
Journal Details
License
Format
Journal
eISSN
2509-8934
First Published
22 Feb 2016
Publication timeframe
1 time per year
Languages
English
Summary

Large - scale isolation of microsatellite and information in any conifer species is limited. Our knowledge of microsatellite in spruce (Picea spp.) is still sketchy. Genomic libraries of P. mariana were constructed and screened with (AC)15 probes. Over 200 positive clones were found for this dinucleotide and ten were analyzed in details. They were sequenced to confirm the presence of microsatellites and to generate polymerase chain reaction (PCR) primers based on sequences flanking the microsatellites. All sequenced (AC)n clones had repeats of n > 23. Primer pairs were designed from seven sequences. These primer pairs along with 15 primer pairs from white spruce (Picea glauca) were tested on individual trees. Seven primer pairs from P. mariana and three from P. glauca (white spruce) amplified DNA from P. mariana and were used for genetic analysis of black spruce populations from uplands (drylands) and lowlands (wetlands). High levels of polymorphism and heterozygosity were observed in all the populations studied. Both highlands and lowlands showed similar levels of genetic variation. The selected microsatellites sequences are being used for genome organization analysis of black spruce.

Keywords

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