1. bookVolume 57 (2008): Issue 1-6 (December 2008)
Journal Details
License
Format
Journal
eISSN
2509-8934
First Published
22 Feb 2016
Publication timeframe
1 time per year
Languages
English
access type Open Access

Discrimination between seedlings of Eucalyptus globulus, E. nitens and their F1 hybrid using near-infrared reflectance spectroscopy and foliar oil content

Published Online: 14 Oct 2017
Volume & Issue: Volume 57 (2008) - Issue 1-6 (December 2008)
Page range: 262 - 269
Received: 05 Jun 2007
Journal Details
License
Format
Journal
eISSN
2509-8934
First Published
22 Feb 2016
Publication timeframe
1 time per year
Languages
English
Summary

Identification of plant hybrids produced from closely related species can be difficult using morphological characteristics alone, particularly when identifying young seedlings. In this study, we compared the performance of three calibration models developed to discriminate between seedlings of Eucalyptus globulus, E. nitens and their first-generation hybrid using either foliar oil chemistry or near-infrared reflectance spectral data from fresh, whole leaves. Both oil and near-infrared reflectance spectroscopy (NIRS) models were developed using partial least-squares discriminant analysis and showed high classification accuracy, all correctly classifying more than 91% of samples in cross-validation. Additionally, we developed a larger, “global” and independently validated NIRS model specifically to discriminate between E. globulus and F1 hybrid seedlings of different ages. This model correctly classified 98.1% of samples in cross-validation and 95.1% of samples from an independent test set. These results show that NIRS analysis of fresh, whole leaves can be used as a rapid and accurate alternative to chemical analysis for the purpose of hybrid identification.

Keywords

ADAMS, R. P. (1989): Identification of Essential Oils by Ion Trap Mass Spectroscopy. Academic Press Inc, San Diego.10.1016/B978-0-12-044230-0.50005-8Search in Google Scholar

ATKINSON, M. D., A. P. JERVIS and R. S. SANGHA (1997): Discrimination between Betula pendula, Betula pubescens, and their hybrids using near-infrared reflectance spectroscopy. Canadian Journal of Forest Research 27, 1896-1900.10.1139/x97-141Open DOISearch in Google Scholar

BARBOUR, R., B. M. POTTS, R. E. VAILLANCOURT, W. N. TIBBITS and R. J. E. WILTSHIRE (2002): Gene flow between introduced and native Eucalyptus species. New Forests 23, 177-191.10.1023/A:1020389432492Open DOISearch in Google Scholar

BARNES, R. J., M. S. DHANOA and S. J. LISTER (1989): Standard normal variate transformation and de-trending of near-infrared diffuse reflectance spectra. Applied Spectroscopy 43, 772-777.10.1366/0003702894202201Open DOISearch in Google Scholar

BATTEN, G. D. (1998): Plant analysis using near infrared reflectance spectroscopy: the potential and the limitations. Australian Journal of Experimental Agriculture 38, 697-706.10.1071/EA97146Open DOISearch in Google Scholar

BERTRAND, B., H. ETIENNE, P. LASHERMES, B. GUYOT and F. DAVRIEUX (2005): Can near-infrared reflectance of green coffee be used to detect introgression in Coffea arabica cultivars? Journal of the Science of Food and Agriculture 85, 955-962.10.1002/jsfa.2049Search in Google Scholar

BOKOBZA, L. (1998): Near infrared spectroscopy. Journal of Near Infrared Spectroscopy 6, 3-17.10.1255/jnirs.116Open DOISearch in Google Scholar

CONZEN, J. P. (2003): Multivariate Calibration: A practical guide for developing methods in the quantitative analytical chemistry. Bruker Optik GmbH, Germany.Search in Google Scholar

COZZOLINO, D., H. E. SMYTH, W. CYNKAR, R. G. DAMBERGS and M. GISHEN (2005): Usefulness of chemometrics and mass spectrometry-based electronic nose to classify Australian white wines by their varietal origin. Talanta 68, 382-387.10.1016/j.talanta.2005.08.057Open DOISearch in Google Scholar

COZZOLINO, D., H. E. SMYTH and M. GISHEN (2003): Feasibility study on the use of visible and near-infrared spectroscopy together with chemometrics to discriminate between commercial white wines of different varietal origins. Journal of Agricultural and Food Chemistry 51, 7703-7708.10.1021/jf034959sOpen DOISearch in Google Scholar

DAVIES, A. M. C. (1998): Cross validation: do we love it too much? Spectroscopy Europe 10, 24-25.Search in Google Scholar

DAVIES, A. M. C. (2001): Uncertainty testing in PLS regression. Spectroscopy Europe 13, 16-19.Search in Google Scholar

DAVIES, N. W. (1990): Gas chromatographic retention indices of monoterpenes and sesquiterpenes on methyl silicone and Carbowax 20M phases. Journal of Chromatography 503, 1-24.10.1016/S0021-9673(01)81487-4Search in Google Scholar

DELAPORTE, K. L., G. G. COLLINS, J. G. CONRAN and M. SEDGLEY (2001): Molecular analysis of an interspecific hybrid ornamental eucalypt for parental identification. Euphytica 122, 165-170.10.1023/A:1012619314419Search in Google Scholar

EBBERS, M., I. R. WALLIS, S. DURY, R. B. FLOYD and W. J. FOLEY (2002): Spectrometric prediction of secondary metabolites and nitrogen in fresh Eucalyptus foliage: towards remote sensing of the nutritional quality of foliage for leaf-eating marsupials. Australian Journal of Botany 50, 761-768.10.1071/BT02025Open DOISearch in Google Scholar

ESPEJO, J., V. HERNANDEZ, V. SIERRA, J. BECERRA and R. LOPEZ (2004): Determination of terpenes through gas chromatography-mass spectrometry (GC-MS) - A new tool in the early determination of hybrid progenies of Eucalyptus nitens x E. globulus. In: Eucalyptus in a changing world. Proc. of IUFRO conf., Aveiro, Portugal. 11-15 OctoberSearch in Google Scholar

ESPEJO, J. E., N. F. ENGLAND and A. R. GRIFFIN (1995): Results of a crossing program with Eucalyptus nitens and E. globulus in Chile. pp. 239-240. In: Eucalypt Plantations: Improving Fibre Yield and Quality. Proc. of CRCTHF-IUFRO conf., Hobart, Tasmania. 19-24 February.Search in Google Scholar

FOLEY, W. J., A. MCILWEE, I. LAWLER, L. ARAGONES, A. P. WOOLNOUGH and N. BERDING (1998): Ecological applications of near infrared reflectance spectroscopy - a tool for rapid, cost-effective prediction of the composition of plant and animal tissues and aspects of animal performance. Oecologia 116, 293-305.10.1007/s00442005059128308060Search in Google Scholar

FRITZ, R. S. (2001): Plant Hybrids. Encyclopedia of Biodiversity 4, 659-675.10.1016/B0-12-226865-2/00344-8Search in Google Scholar

GELADI, P., H. MARTENS, L. HADJIISKI and P. HOPKE (1996): A calibration tutorial for spectral data. Part 2. Partial least squares regression using Matlab and some neural network results. Journal of Near Infrared Spectroscopy 4, 243-255.10.1255/jnirs.94Open DOISearch in Google Scholar

GILLON, D., C. HOUSSARD and R. JOFFRE (1999): Using near-infrared reflectance spectroscopy to predict carbon, nitrogen and phosphorus content in heterogeneous plant material. Oecologia 118, 173-182.10.1007/s00442005071628307692Open DOISearch in Google Scholar

GIVENS, D. I., J. L. DE BOEVER and E. R. DEAVILLE (1997): The principles, practices and some future applications of near infrared spectroscopy for predicting the nutritive value of foods for animals and humans. Nutrition Research Reviews 10, 83-114.10.1079/NRR1997000619094259Search in Google Scholar

GORE, P. L., B. M. POTTS, P. W. VOLKER and J. MEGALOS (1990): Unilateral cross-incompatibility in Eucalyptus: the case of hybridisation between E. globulus and E. nitens. Australian Journal of Botany 38, 383-394.10.1071/BT9900383Open DOISearch in Google Scholar

GRAYLING, P. M. and M. I. H. BROOKER (1996): Evidence for the identity of the hybrid Eucalyptus ‘brachyphylla’ (Myrtaceae) from morphology and essential-oil composition. Australian Journal of Botany 44, 1-13.10.1071/BT9960001Open DOISearch in Google Scholar

GRIFFIN, A. R., I. P. BURGESS and L. WOLF (1988): Patterns of natural and manipulated hybridization in the genus Eucalyptus L’Herit.: a review. Australian Journal of Botany 36, 41-66.10.1071/BT9880041Search in Google Scholar

GRIFFIN, A. R., J. L. HARBARD, C. CENTURION and P. SANTINI (2000): Breeding Eucalyptus grandis x globulus and other inter-specific hybrids with high inviability - problem analysis and experience with Shell Forestry projects in Uruguay and Chile. pp. 1-13. In: Hybrid Breeding and Genetics of Forest Trees. Proc. of QFRI/CRCSPF Symposium, 9-14th April, Queensland, Australia.Search in Google Scholar

LAWLER, I. R., W. J. FOLEY and B. M. ESCHLER (2000): Foliar concentration of a single toxin creates habitat patchiness for a marsupial folivore. Ecology 81, 1327-1338.10.1890/0012-9658(2000)081[1327:FCOAST]2.0.CO;2Open DOISearch in Google Scholar

LISTER, S. J., M. S. DHANOA, J. L. STEWART and M. GILL (2000): Classification and comparison of Gliricidia provenances using near infrared reflectance spectroscopy. Animal Feed Science and Technology 86, 221-238.10.1016/S0377-8401(00)00165-6Open DOISearch in Google Scholar

LONEY, P. E. (2007): Links between ontogeny, chemical and physical characteristics of foliage and mammalian herbivory in Eucalyptus nitens. Unpublished PhD Thesis, University of Tasmania.Search in Google Scholar

MARTIN, B. (1989): The benefits of hybridization. How do you breed for them? pp. 79-92. In: Population structure and genetic improvement strategies in clonal and seedling forestry. Proc. of the IUFRO conf. on breeding tropical trees, Pattaya, Thailand. November 1988.Search in Google Scholar

MOLLER, B. (2004): Near infrared transmission spectra of barley of malting grade represent a physical-chemical fingerprint of the sample that is able to predict germinative vigour in a multivariate data evaluation model. Journal of the Institute of Brewing 110, 18-33.10.1002/j.2050-0416.2004.tb00177.xSearch in Google Scholar

MORON, A., A. GARCIA, J. SAWCHIK and D. COZZOLINO (2007): Preliminary study on the use of near-infrared reflectance spectroscopy to assess nitrogen content of undried wheat plants. Journal of the Science of Food and Agriculture 87, 147-152.10.1002/jsfa.2691Search in Google Scholar

NAES, T., T. ISAKSSON, T. FEARN and T. DAVIES (2002): A user friendly guide to multivariate calibration and classification. NIR Publications, Chichester, UK.Search in Google Scholar

NIST/EPA/NIH Mass Spectral Data Base, Version 2.0a. U.S. Department of Commerce (2002).Search in Google Scholar

O’REILLY-WAPSTRA, J. M., C. MCARTHUR and B. M. POTTS (2004): Linking plant genotype, plant defensive chemistry and mammal browsing in a Eucalyptus species. Functional Ecology 18, 677-684.10.1111/j.0269-8463.2004.00887.xOpen DOISearch in Google Scholar

OURCIVAL, J. M., R. JOFFRE and S. RAMBAL (1999): Exploring the relationships between reflectance and anatomical and biochemical properties in Quercus ilex leaves. New Phytologist 143, 351-364.10.1046/j.1469-8137.1999.00456.xSearch in Google Scholar

PARK, R. S., R. E. AGNEW, F. J. GORDON and R. W. J. STEEN (1998): The use of near infrared reflectance spectroscopy (NIRS) on undried samples of grass silage to predict chemical composition and digestibility parameters. Animal Feed Science and Technology 72, 155-167.10.1016/S0377-8401(97)00175-2Search in Google Scholar

POKE, F. S. and C. A. RAYMOND (2006): Predicting extractives, lignin, and cellulose contents using near infrared spectroscopy on solid wood in Eucalyptus globulus. J. Wood Chem. Technol. 26, 187-199.10.1080/02773810600732708Open DOISearch in Google Scholar

POTTS, B. M., R. C. BARBOUR, A. HINGSTON and R. E. VAILLANCOURT (2003): Turner Review No. 6: Genetic pollution of native eucalypt gene pools - identifying the risks. Australian Journal of Botany 51, 1-25.10.1071/BT02035Open DOISearch in Google Scholar

POTTS, B. M. and H. S. DUNGEY (2004): Interspecific hybridization of Eucalyptus: key issues for breeders and geneticists. New Forests 27, 115-138.10.1023/A:1025021324564Open DOISearch in Google Scholar

POTTS, B. M., R. E. VAILLANCOURT, G. J. JORDAN, G. W. DUTKOWSKI, J. COSTA E. SILVA, G. E. MCKINNON, D. A. STEANE, P. W. VOLKER, G. A. LOPEZ, L. A. APIOLAZA, Y. LI, C. MARQUES and N. M. G. BORRALHO (2004): Exploration of the Eucalyptus globulus gene pool. pp. 46-61. In: Eucalyptus in a changing world. Proc. of IUFRO conf., Aveiro, Portugal. 11-15 October.Search in Google Scholar

POTTS, B. M., P. W. VOLKER, P. A. TILYARD and K. JOYCE (2000): The genetics of hybridisation in the temperate Eucalyptus. pp. 200-211. In: Hybrid Breeding and Genetics of Forest Trees. Proc. of QFRI/CRC-SPF Symposium, 9-14th April, Queensland, Australia.Search in Google Scholar

POTTS, B. M. and R. J. E. WILTSHIRE (1997): Eucalypt genetics and genecology. In ‘Eucalypt Ecology: Individuals to Ecosystems’. (Ed. J. W. a. J. WOINARSKI.) Cambridge University Press, Cambridge.Search in Google Scholar

PRYOR, L. D. (1976): The Biology of Eucalypts. Studies in Biology. Edward Arnold, London.Search in Google Scholar

PRYOR , L. D. and L. A. S. JOHNSON (1981): Eucalyptus, the universal Australian. In ‘Ecological Biogeography of Australia’. (Ed. A. KEAST.) pp. 499-536. W. Junk, The Hague.10.1007/978-94-009-8629-9_17Search in Google Scholar

RICHARDSON, A. D., J. B. REEVES and T. G. GREGOIRE (2003): Multivariate analyses of visible/near infrared (VIS/NIR) absorbance spectra reveal underlying spectral differences among dried, ground conifer needle samples from different growth environments. New Phytologist 161, 291-301.10.1046/j.1469-8137.2003.00913.xSearch in Google Scholar

SALE, M., B. M. POTTS, A. WEST and J. REID (1996): Molecular differentiation within and between Eucalyptus risdonii, E. amygdalina and their hybrids using RAPD markers. Australian Journal of Botany 44, 559-569.10.1071/BT9960559Open DOISearch in Google Scholar

SCHIMLECK, L. R., A. J. MICHELL and P. VINDEN (1996): Eucalypt wood classification by NIR spectroscopy and principal components analysis. Appita Journal 49, 319-324.Search in Google Scholar

STOLTER, C., R. JULKUNEN-TIITTO and J. U. GANZHORN (2006): Application of near infrared reflectance spectroscopy (NIRS) to assess some properties of a sub-arctic ecosystem. Basic and Applied Ecology 7, 167-187.10.1016/j.baae.2005.05.002Open DOISearch in Google Scholar

TIBBITS, W. N. (1988): Germination and morphology of progeny from controlled pollinations of Eucalyptus nitens (Deane and Maiden) Maiden. Australian journal of botany 36, 677-691.10.1071/BT9880677Open DOISearch in Google Scholar

TIBBITS, W. N. (1989): Controlled pollination studies with shining gum (Eucalyptus nitens (Deane and Maiden) Maiden). Forestry 62, 111-126.10.1093/forestry/62.2.111Search in Google Scholar

TIBBITS, W. N., D. B. BOOMSMA and, S. JARVI (1997): Distribution, biology, genetics, and improvement programs for Eucalyptus globulus and E. nitens around the world. pp. 1-15. In: Proc. of the 24th Biennial Southern Tree Improvement Conf., June 9-12, Orlando, Florida.Search in Google Scholar

TIBBITS, W. N., B. M. POTTS and M. H. SAVVA (1991): Inheritance of freezing resistance in interspecific F1 hybrids of Eucalyptus. Theoretical and Applied Genetics 83, 126-135.10.1007/BF0022923524202266Search in Google Scholar

TIGABU, M. and P. C. ODEN (2003): Discrimination of viable and empty seeds of Pinus patula Schiede & Deppe with near-infrared spectroscopy. New Forests 25, 163-176.10.1023/A:1022916615477Open DOISearch in Google Scholar

TIGABU, M., P. C. ODEN and T. Y. SHEN (2004): Application of near-infrared spectroscopy for the detection of internal insect infestation in Picea abies seed lots. Canadian Journal of Forest Research 34, 76-84.10.1139/x03-189Search in Google Scholar

TIGABU, M., P. C. ODEN and D. LINDGREN (2005): Identification of seed sources and parents of Pinus sylvestris L. using visible-near infrared reflectance spectra and multivariate analysis. Trees - Structure and Function 19, 468-476.10.1007/s00468-005-0408-5Search in Google Scholar

VILJOEN, M., L.C. HOFFMAN and T. S. Brand (2007): Prediction of the chemical composition of mutton with near infrared reflectance spectroscopy. Small Ruminant Research 69, 88-94.10.1016/j.smallrumres.2005.12.019Search in Google Scholar

VOLKER, P. (2002): Quantitative genetics of Eucalyptus globulus, E. nitens and their F1 hybrid. Unpublished PhD Thesis, Univeristy of Tasmania.Search in Google Scholar

VOLKER, P. W. (1995): Evaluation of Eucalyptus nitens x globulus for commercial forestry. pp. 222-225. In: Proc. of the CRCTHF-IUFRO Conf., Hobart, Tasmania. 19-24 February.Search in Google Scholar

WOODCOCK, T., G. DOWNEY, J. D. KELLY, C. O’DONNELL (2007): Geographical classification of honey samples by near-infrared spectroscopy: A feasibility study. Journal of Agricultural and Food Chemistry 55, 9128-9134.10.1021/jf072010q17927137Open DOISearch in Google Scholar

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