<abstract xmlns="http://www.w3.org/1999/xhtml"><p> Molecular evidence for spontaneous hybridization between Pinus sylvestris L. and P. mugo Turra in the putative hybrid swarm populations of the species in Slovakia was provided based on PCR-RFLP analysis of the cpDNA trnV-trnH region. Species-specific restriction profiles generated by Hinf I digests of the cpDNA products reliably identified P. sylvestris and P. mugo haplotypes of the embryos from open pollination. Simultaneous analysis of the respective cpDNA region in megagametophytes and embryos of individual seeds along with needles of a given maternal tree has enabled to score either the P. sylvestris or P. mugo haplotypes in the embryos illustrating hybridization patterns between the two species. Data obtained in this way indicate a relatively extensive hybridization which takes place between P. sylvestris and P. mugo. The extent of hybridization varied among populations as evidenced by the 41.1-58.7% proportion of hybrid embryos registered on the locality Habovka, and by the 8.3% and 2.7% proportions of hybrid embryos on the localities Tisovnica and Sucha Hora, respectively. The approach itself is recommended as a convenient method for monitoring the hybridization patterns in sympatric zones of the studied pine species. </p></abstract>

Molecular evidence for spontaneous hybridization between Pinus sylvestris L. and P. mugo Turra in the putative hybrid swarm populations of the species in Slovakia was provided based on PCR-RFLP analysis of the cpDNA trnV-trnH region. Species-specific restriction profiles generated by Hinf I digests of the cpDNA products reliably identified P. sylvestris and P. mugo haplotypes of the embryos from open pollination. Simultaneous analysis of the respective cpDNA region in megagametophytes and embryos of individual seeds along with needles of a given maternal tree has enabled to score either the P. sylvestris or P. mugo haplotypes in the embryos illustrating hybridization patterns between the two species. Data obtained in this way indicate a relatively extensive hybridization which takes place between P. sylvestris and P. mugo. The extent of hybridization varied among populations as evidenced by the 41.1-58.7% proportion of hybrid embryos registered on the locality Habovka, and by the 8.3% and 2.7% proportions of hybrid embryos on the localities Tisovnica and Sucha Hora, respectively. The approach itself is recommended as a convenient method for monitoring the hybridization patterns in sympatric zones of the studied pine species.

Spontaneous Hybridization between Pinus sylvestris L. and P. mugo Turra in Slovakia

Silvae Genetica

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 License.

Molecular evidence for spontaneous hybridization between Pinus sylvestris L. and P. mugo Turra in the putative hybrid swarm populations of the species in...

Spontaneous Hybridization between Pinus sylvestris L. and P. mugo Turra in Slovakia

Published Online: Oct 14, 2017

Page range: 76 - 82

Received: Nov 28, 2006

DOI: https://doi.org/10.1515/sg-2008-0012

Keywords
Pinus sylvestris L., P. mugo Turra, hybridization, chloroplast DNA, restriction analysis

© 2017

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 License.

Spontaneous Hybridization between Pinus sylvestris L. and P. mugo Turra in Slovakia

Published Online: Oct 14, 2017

Page range: 76 - 82

Received: Nov 28, 2006

DOI: https://doi.org/10.1515/sg-2008-0012

KeywordsPinus sylvestris L., P. mugo Turra, hybridization, chloroplast DNA, restriction analysis

© 2017

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 License.

Keywords
Pinus sylvestris L., P. mugo Turra, hybridization, chloroplast DNA, restriction analysis