1. bookVolume 57 (2008): Issue 1-6 (December 2008)
Journal Details
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Format
Journal
eISSN
2509-8934
First Published
22 Feb 2016
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1 time per year
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English
access type Open Access

Construction of a Populus tremuloides Michx. BAC library

Published Online: 14 Oct 2017
Volume & Issue: Volume 57 (2008) - Issue 1-6 (December 2008)
Page range: 65 - 69
Received: 13 Nov 2006
Journal Details
License
Format
Journal
eISSN
2509-8934
First Published
22 Feb 2016
Publication timeframe
1 time per year
Languages
English
Abstract

We have constructed an aspen (Populus tremuloides Michx., line Turesson141) BAC library containing 55,296 clones in total. A random sampling of 86 BAC clones indicated an average insert size of 76 kb with a range of 20 to 160 kb. Twelve percent of the BAC clones in the library have an insert size larger than 100 kb. Based on an estimated genome size for Populus of 500 Mbp, library coverage is about 8 haploid genome equivalents. This library will be screened using AFLP marker identified before co-segregating with gender in a P. tremula x P. tremuloides progeny, where Turesson141 was the male parent.

Keywords

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