1. bookVolume 56 (2007): Issue 1-6 (December 2007)
Journal Details
License
Format
Journal
eISSN
2509-8934
First Published
22 Feb 2016
Publication timeframe
1 time per year
Languages
English
access type Open Access

Characterization of a Suite of 40 EST-derived Microsatellite Markers For Use in Sitka Spruce (Picea sitchensis (Bong.) Carr)

Published Online: 14 Oct 2017
Volume & Issue: Volume 56 (2007) - Issue 1-6 (December 2007)
Page range: 138 - 141
Received: 04 Apr 2006
Journal Details
License
Format
Journal
eISSN
2509-8934
First Published
22 Feb 2016
Publication timeframe
1 time per year
Languages
English
Abstract

This paper describes 40 novel, data-mined, polymorphic microsatellite loci for use in a QTL association study in Sitka spruce. Publicly available EST sequences of Picea in Genbank were searched in silico for simple sequence repeat (SSR) motifs, principally dinucleotide microsatellites, and PCR primers were designed to flank these regions. PCR amplification was carried out in the progeny of a full-sib family to test simple Mendelian inheritance. For further characterization, the amplification products of Sitka spruce material from unrelated trees were assessed to determine the potential of these loci for population genetic studies. These polymorphic markers therefore represent a valuable tool-kit both for establishing a molecular map of this species and for Picea genetic population studies.

Keywords

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