1. bookVolume 55 (2006): Issue 1-6 (December 2006)
Journal Details
License
Format
Journal
eISSN
2509-8934
First Published
22 Feb 2016
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1 time per year
Languages
English
access type Open Access

Identification of European and Japanese Larch and Their Interspecific Hybrid with Morphological Markers: Application to Young Seedlings

Published Online: 19 Oct 2017
Volume & Issue: Volume 55 (2006) - Issue 1-6 (December 2006)
Page range: 123 - 134
Received: 30 Nov 2005
Journal Details
License
Format
Journal
eISSN
2509-8934
First Published
22 Feb 2016
Publication timeframe
1 time per year
Languages
English
Abstract

Open-pollinated hybridisation seed orchards of European and Japanese larches produce mixed progenies combining a highly variable proportion of hybrids along with pure parental species. For several reasons, it is desirable to identify and to sort out hybrids from pure species at the seedling stage. Taxa identification of 1-2 yr-old seedlings was attempted using non-destructive assessment of several traits, including morphology, phenology, growth and architecture parameters. Two sets of progenies originating from 10 open-pollinated hybridisation seed orchards were used, relying in a first step on taxa identification of individual seedlings with diagnostic molecular markers. Based on 21 traits assessed, some clear trends in pure species and hybrid features were apparent but due to the large and overlapping ranges of taxa characteristics, no single parameter allowed unambiguous identification of taxa. Combination of traits through linear discriminant analysis made possible correct classification of 90.2% to 98.6% of individuals depending on the orchard although there were a few problematic orchards. Two traits appeared particularly pertinent for discriminating young plants taxa, namely 1st-yr leaf retention (marcescence) and the bark colour of 2nd-year shoot increments. Results were corroborated using progenies from several orchards and over two experimental periods.

Keywords

ACHERÉ, V. (1999): Recherche de marqueurs moléculaires pour l’identification des mélèzes hybrides (Larix x eurolepis). Mémoire DEA Univ.Nancy I, 20 pp.Search in Google Scholar

ACHERÉ, V., P. FAIVRE RAMPANT, L. E. PÂQUES and D. PRAT (2004): Chloroplast and mitochondrial molecular tests identify European-Japanese larch hybrids. Theor. Appl. Genet. 108: 1643-1649.Search in Google Scholar

BALTUNIS, B. S., M. S. GREENWOOD and T. EYSTEINSSON (2000): Hybrid vigor in Larix: growth of intra- and interspecific hybrids of Larix decidua, L. laricina and L. kaempferi after 5-years. Silvae Genet. 47: 288-293.Search in Google Scholar

BERGMANN, F. and W. RUETZ (1987): Identifizierung von Hybridlärchensaatgut aus Samenplantagen mit Hilfe eines Isoenzym-Markers. Silvae Genet. 36 (2): 102-105.Search in Google Scholar

BRAUN, H. (1990): Untersuchungen zum Anteil von Hybridlärchen aus einer Samenplantage. Beotr. Forstwirtschaft 24: 151-155.Search in Google Scholar

DEBAZAC, E. F. (1964): Manuel des Conifères. Ed. Ecole Nationale des Eaux et Forêts, Nancy.Search in Google Scholar

DELEVOY, G. (1949): Larix eurolepis. Bull. Soc. Centr. Forest. Belgique 56: 178-194.Search in Google Scholar

DESTEUCQ, O. (2003): Catalogue of Larch basic materials in Europe. ‘Towards a European Larch Wood Chain’ EU project. Ed. Centre de Recherche de la Nature, des Forêts et du Bois, Gembloux (B).Search in Google Scholar

ENNOS, R. A. and T. QIAN (1994): Monitoring the output of a hybrid larch seed orchard using isozymes markers. Forestry 67: 63-74.10.1093/forestry/67.1.63Open DOISearch in Google Scholar

GROS-LOUIS, M. C., J. BOUSQUET, L. E. PÂQUES and N. ISABEL (2005): Species-diagnostic markers in Larix spp. based on RAPDs and nuclear, cpDNA and mtDNA gene sequences, and their phylogenetic implications. Theor. Appl. Genet. Tree Genetics & Genome 1 (2): 50-63.Search in Google Scholar

HÄCKER, M. and F. BERGMANN (1991): The proportion of hybrids in seed from seed orchard composed of two larch species (L. europaea and L. leptolepis). Ann. Sci. For. 48: 631-640.10.1051/forest:19910602Open DOISearch in Google Scholar

HENRY, A. and M. G. FLOOD (1919): The history of the Dunkeld larch. Proc. Royal Irish Acad. Sec. B. 35: p55 in Litterfield & Eliason.Search in Google Scholar

HINCKLEY, T. M., R. CEULEMANS, J. M. DUNLAP, A. FIGLIOLA, P. E. HEILMAN, J. G. ISEBRANDS, G. SCARASCIAMUGNOZZA, P. J. SCHULTE, B. SMIT, R. F. STETTLER, E. VAN VOLKENBURGH and M. WIARD (1989): Physiological, morphological and anatomical components of hybrid vigor in Populus. In: Structural and functional responses to environmental stresses. Edited by K. H. KREEB, H. RICHTER and T. M. HINCKLEY. SPB Academic Publishing, The Hague, pp. 199-217.Search in Google Scholar

HUBERTY, C. J. (1994): Applied discriminant analysis. Wiley-Intesciences (Wiley series in Probability and Statistics), New-York. Search in Google Scholar

KLEINSCHMIT, J. R. G., R. BACILIERI, A. KREMER and A. ROLOFF (1995): Comparison of morphological traits of pedunculate oak (Q. robur L.) and sessile oak (Q. petraea (Matt.) Liebl.). Silvae Genet. 44: 256-269.Search in Google Scholar

KREMER, A., J. L. DUPOUEY, J. COTTRELL, U. CSAIKL, D. DEANS, R. FINKELDEY, P. GOIKOETXEA, J. JENSEN, A. KÖNIG, B. VAN DAM, A. DUCOUSSO and V. BADEAU (2002): Morphological variation in mixed oak stands (Quercus robur and Quercus petraea) is stable across European populations. Ann. For. Sci. 59: 777-787.Search in Google Scholar

LAING, E.V. (1944): Studies on the genus Larix with particular reference to the hybrid larch. Scottish Forestry Journal 58: 6- 32.Search in Google Scholar

LALLY, M. and D. THOMPSON (1998): The advantages of hybrid over European and Japanese larch. Coillte Research and Development, Information note n° 16.Search in Google Scholar

LITTLE, D. P. (2004): Documentation of hybridization between Californian cypresses: Cupressus macnabiana x sargentii. Systematic Botany 29: 825-833.10.1600/0363644042451026Search in Google Scholar

MAYR, E. (1996): What is a Species, and What is Not? Philosophy of Science 63: 262-277. Munsell color charts for plant tissues, Munsell color New York.10.1086/289912Open DOISearch in Google Scholar

NKONGOLO, K. K. and K. KLIMASZWSKA (1995): Cytological and molecular relationships between Larix decidua, L. leptolepis and Larix x eurolepis: identification of species-specific chromosoms and synchronisation of mitotic cells. Theor. Appl. Genet. 90: 827-834.Search in Google Scholar

OSTENFELD, C. H. and C. S. LARSEN (1930): The species of the genus Larix and their geographic distribution. Biol. Meddel. Kongel. Danske Vidensk. Selsk. 9: 1-107.Search in Google Scholar

PÂQUES, L. E. (2000): Interspecific hybridisation in larch: the long way to get outstanding varieties. In: HybridBreeding and Genetics of Forest Trees. DUNGEY, H. S., DIETERS, M. J. and NIKLES, D. G. (Compilers). Proc. of QFRI/CRC-SPF Symposium, 9-14 April 2000, Noosa, Queensland, Australia, pp. 373-385.Search in Google Scholar

PÂQUES, L. E. (2002): Larch tree improvement programme in France. In: Proceedings of the LARIX 2002 symposium, Gap, France, 16-21 Sept.2002; Ed. INRA Orléans, pp. 104-118.Search in Google Scholar

PATSCHKE, W. (1913): Über die extratropischen ostasiatischen Coniferen und ihre Bedeutung für die pflanzengeographische Gliederung Ostasiens. Bot. Jahrb. Syst. 48: 626-776.Search in Google Scholar

PONTON, S., J. L. DUPOUEY and E. DREYER (2004): Leaf morphology as species indicator in seedlings of Quercus robur L. and Q. petraea (Matt.) Liebl.: modulation by irradiance and growth flush. Ann. For. Sci. 61: 73-80.Search in Google Scholar

QIAN, T., R. ENNOS and T. HELGASON (1995): Genetic relationships among larch species based on analysis of restriction fragment variation for chloroplast DNA. Can. J. For. Res. 25: 1197-1202.Search in Google Scholar

RIESEBERG, L. H. and N. C. ELLSTRAND (1993): What can molecular and morphological markers tell us about plant hybridization? Critical Reviews in Plant Sciences 12 (4): 213-241.10.1080/07352689309701902Search in Google Scholar

SAPORTA, G. (1990): Probabilités, analyse des données et statistique. Ed. Technip. Paris.Search in Google Scholar

SCHEEPERS, D., M. C. ELOY and M. BRIQUET (2000): Identification of larch species (Larix decidua, Larix kaempferi and Larix x eurolepis) and estimation of hybrid fraction in seed lots by RAPD fingerprints. Theor. Appl. Genet. 100 (1): 71-74.10.1007/s001220050010Search in Google Scholar

SEMERIKOV, V. L., H. ZHANG, M. SUN and M. LASCOUX (2003): Conflicting phylogenies of Larix (Pinaceae) based on cytoplasmic and nuclear DNA. Molecular Phylogenetics and Evolution 27: 173-184.10.1016/S1055-7903(02)00447-5Open DOISearch in Google Scholar

SEMERIKOV, V. L., L. F. SEMERIKOV and M. LASCOUX (1999): Intra- and interspecific allozyme variability in eurasian Larix Mill. species. Heredity 82: 193-204.10.1038/sj.hdy.6884710Open DOISearch in Google Scholar

STECCONI, M., P. MARCHELLI, J. PUNTIERI, P. PICCA and L. GALLO (2004): Natural Hybridization between a Deciduous (Nothofagus antarctica, Nothofagaceae) and an Evergreen (N. dombeyi) Forest Tree Species: Evidence from Morphological and Isoenzymatic Traits. Ann. Bot. 94: 775-786.10.1093/aob/mch205424227215489253Search in Google Scholar

VENDRAMIN, G. G., L. LELLI, P. ROSSI and M. MORGANTE (1996): A set of primers for the amplification of 20 chloroplast microsatellites in Pinaceae. Molecular Ecology 5: 595-598.10.1111/j.1365-294X.1996.tb00353.x8794566Open DOISearch in Google Scholar

WEI, X. X. and X. Q. WANG (2003): Phylogenetic split of Larix: evidence from paternally inherited cpDNA trnTtrnF region. Plant Systematics and Evolution 239: 67-77.10.1007/s00606-002-0264-3Search in Google Scholar

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