<abstract xmlns="http://www.w3.org/1999/xhtml"><p> The Chinese plant, “Packchul”, (Atractylodes japonica or A. macrocephala), is a very important Chinese medicinal herb plant and is called Sabju in Korea. The levels of the active components are different between these two species, but these medicines are sold in Korean herbal markets without discrimination. This study was carried out to develop a method that could be used to discriminate between A. japonica and A. macrocephala based on molecular markers. To discriminate between A. japonica and A. macrocephala, RAPD analyses were used to develop SCAR markers. Eighteen species-specific RAPD bands were obtained from 52 OPERON and URP primer sets. Two SCAR markers were developed from these RAPD clones. Both SCAR markers were cloned into pGEM-T-Easy vectors and then subjected to nucleotide sequence analysis and designated AjR1 (1,117 bp) and AmR1 (1,325 bp). These two markers were sufficient to discriminate between samples of A. japonica and A. macrocephala.</p></abstract>

The Chinese plant, “Packchul”, (Atractylodes japonica or A. macrocephala), is a very important Chinese medicinal herb plant and is called Sabju in Korea. The levels of the active components are different between these two species, but these medicines are sold in Korean herbal markets without discrimination. This study was carried out to develop a method that could be used to discriminate between A. japonica and A. macrocephala based on molecular markers. To discriminate between A. japonica and A. macrocephala, RAPD analyses were used to develop SCAR markers. Eighteen species-specific RAPD bands were obtained from 52 OPERON and URP primer sets. Two SCAR markers were developed from these RAPD clones. Both SCAR markers were cloned into pGEM-T-Easy vectors and then subjected to nucleotide sequence analysis and designated AjR1 (1,117 bp) and AmR1 (1,325 bp). These two markers were sufficient to discriminate between samples of A. japonica and A. macrocephala.

Identification of Atractylodes japonica and A. macrocephala by RAPD analysis and SCAR Markers

Silvae Genetica

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 License.

The Chinese plant, “Packchul”, (Atractylodes japonica or A. macrocephala), is a very important Chinese medicinal herb plant and is called Sabju in Korea. The...

Identification of Atractylodes japonica and A. macrocephala by RAPD analysis and SCAR Markers

Published Online: Oct 19, 2017

Page range: 101 - 105

Received: Apr 24, 2005

DOI: https://doi.org/10.1515/sg-2006-0015

Keywords
Atractylodes japonica, A. macrocephala, RAPD, SCAR

© 2017

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 License.

Identification of Atractylodes japonica and A. macrocephala by RAPD analysis and SCAR Markers

Published Online: Oct 19, 2017

Page range: 101 - 105

Received: Apr 24, 2005

DOI: https://doi.org/10.1515/sg-2006-0015

KeywordsAtractylodes japonica, A. macrocephala, RAPD, SCAR

© 2017

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 License.

Keywords
Atractylodes japonica, A. macrocephala, RAPD, SCAR