<abstract xmlns="http://www.w3.org/1999/xhtml"><p> The major drawback of microsatellites analysis is that they are expensive to develop, labor-intensive and demand skilled personnel. However, such studies might be still simplified and accelerated by multiplexing of the markers and the use of highthroughput systems for genotyping DNA fragments. In this paper we present a single, simple and highly effective PCRmultiplex reaction composed of six chloroplast microsatellites widely used for population studies in pines but here applied to Pinus sylvestris. The reaction allows for rapid genotyping of large sample sizes.</p></abstract>

The major drawback of microsatellites analysis is that they are expensive to develop, labor-intensive and demand skilled personnel. However, such studies might be still simplified and accelerated by multiplexing of the markers and the use of highthroughput systems for genotyping DNA fragments. In this paper we present a single, simple and highly effective PCRmultiplex reaction composed of six chloroplast microsatellites widely used for population studies in pines but here applied to Pinus sylvestris. The reaction allows for rapid genotyping of large sample sizes.

PCR-Multiplex of Six Chloroplast Microsatellites for Population Studies and Genetic Typing in Pinus sylvestris

Silvae Genetica

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 License.

The major drawback of microsatellites analysis is that they are expensive to develop, labor-intensive and demand skilled personnel. However, such studies might...

PCR-Multiplex of Six Chloroplast Microsatellites for Population Studies and Genetic Typing in Pinus sylvestris

Published Online: Oct 27, 2017

Page range: 246 - 248

Received: Aug 19, 2004

DOI: https://doi.org/10.1515/sg-2004-0045

Keywords
chloroplast microsatellites, PCR-multiplex, Pinus sylvestris, polymorphism, haplotypes

© 2017

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 License.

PCR-Multiplex of Six Chloroplast Microsatellites for Population Studies and Genetic Typing in Pinus sylvestris

Published Online: Oct 27, 2017

Page range: 246 - 248

Received: Aug 19, 2004

DOI: https://doi.org/10.1515/sg-2004-0045

Keywordschloroplast microsatellites, PCR-multiplex, Pinus sylvestris, polymorphism, haplotypes

© 2017

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 License.

Keywords
chloroplast microsatellites, PCR-multiplex, Pinus sylvestris, polymorphism, haplotypes