<abstract xmlns="http://www.w3.org/1999/xhtml"><p> Ten microsatellite loci were used to DNA profile 215 first and second generation breeding clones in the Australian radiata pine breeding program. The clones were essentially unique from one another and these DNA profiles were used to examine the sexual and asexual phases in the breeding program. Only 2.6% of ramets from 25 breeding clones were incorrect whereas 8.4% of progeny from 10 families were genetically incorrect. The lineages of second generation clones were checked for DNA profiles that were available for putative parents. Some parents appear to be incorrect on the basis of the DNA profiles and further sampling will be required to confirm these results across the breeding program. Microsatellite markers can now enable most questions to be resolved in breeding programs and also will allow novel breeding strategies to be used in the future.</p></abstract>

Ten microsatellite loci were used to DNA profile 215 first and second generation breeding clones in the Australian radiata pine breeding program. The clones were essentially unique from one another and these DNA profiles were used to examine the sexual and asexual phases in the breeding program. Only 2.6% of ramets from 25 breeding clones were incorrect whereas 8.4% of progeny from 10 families were genetically incorrect. The lineages of second generation clones were checked for DNA profiles that were available for putative parents. Some parents appear to be incorrect on the basis of the DNA profiles and further sampling will be required to confirm these results across the breeding program. Microsatellite markers can now enable most questions to be resolved in breeding programs and also will allow novel breeding strategies to be used in the future.

DNA Profiling, Pedigree Lineage Analysis and Monitoring in the Australian Breeding Program of Radiata Pine

Silvae Genetica

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 License.

Ten microsatellite loci were used to DNA profile 215 first and second generation breeding clones in the Australian radiata pine breeding program. The clones...

DNA Profiling, Pedigree Lineage Analysis and Monitoring in the Australian Breeding Program of Radiata Pine

Published Online: Oct 27, 2017

Page range: 130 - 134

Received: Aug 11, 2004

DOI: https://doi.org/10.1515/sg-2004-0023

Keywords
DNA fingerprinting, microsatellite loci, probability of identity, paternity

© 2017

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 License.

DNA Profiling, Pedigree Lineage Analysis and Monitoring in the Australian Breeding Program of Radiata Pine

Published Online: Oct 27, 2017

Page range: 130 - 134

Received: Aug 11, 2004

DOI: https://doi.org/10.1515/sg-2004-0023

KeywordsDNA fingerprinting, microsatellite loci, probability of identity, paternity

© 2017

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 License.

Keywords
DNA fingerprinting, microsatellite loci, probability of identity, paternity