<abstract xmlns="http://www.w3.org/1999/xhtml"><p> Objective: The aim of this research is to evaluate the value of the histological score based on a histological record compared to the histometry for monitoring cranial bone defect healing. Methods: We designed a case -control study with a control and a study group. For a number of 60 CD1 mice representing the study group, a bone defect in the cranial bone was surgically induced and grafted with bone grafts obtained by tissue engineering. Bone grafts were obtained using embryonic stem cells seeded on a scaffold obtained from the red deer antler, and osteogenic basal and complex medium was used as differentiation medium. For other 30 CD1 mice representing the control group, a bone defect in the cranial bone was induced and left to heal without grafts. The regeneration process was assessed after 2 and 4 months using the histological healing scoring system and histometry. Results: The healing score was statistically significantly correlated with the defect size obtained by means of histometry (p&lt;0.001). The evaluation of the parameters comprised in the healing score shows that regeneration of the bone diastasis was the most advanced in the group sacrificed at 4 months after plasty, which employed embryonic stem cells, a complex osteogenic differentiation medium and deer antler as scaffold. Conclusion: histological method based on a histological score is a valuable quantification system of bone regeneration comparable to histometry. Clinical Relevance: This study proves that the presented histological score can help the clinician in the process of bone regeneration evaluation.</p></abstract>

Objective: The aim of this research is to evaluate the value of the histological score based on a histological record compared to the histometry for monitoring cranial bone defect healing. Methods: We designed a case -control study with a control and a study group. For a number of 60 CD1 mice representing the study group, a bone defect in the cranial bone was surgically induced and grafted with bone grafts obtained by tissue engineering. Bone grafts were obtained using embryonic stem cells seeded on a scaffold obtained from the red deer antler, and osteogenic basal and complex medium was used as differentiation medium. For other 30 CD1 mice representing the control group, a bone defect in the cranial bone was induced and left to heal without grafts. The regeneration process was assessed after 2 and 4 months using the histological healing scoring system and histometry. Results: The healing score was statistically significantly correlated with the defect size obtained by means of histometry (p<0.001). The evaluation of the parameters comprised in the healing score shows that regeneration of the bone diastasis was the most advanced in the group sacrificed at 4 months after plasty, which employed embryonic stem cells, a complex osteogenic differentiation medium and deer antler as scaffold. Conclusion: histological method based on a histological score is a valuable quantification system of bone regeneration comparable to histometry. Clinical Relevance: This study proves that the presented histological score can help the clinician in the process of bone regeneration evaluation.

Comparative assessment of bone regeneration by histometry and a histological scoring system / Evaluarea comparativă a regenerării osoase utilizând histometria și un scor de vindecare histologică

University of Medicine and Pharmacy “Iuliu Hațieganu” Cluj Napoca

“Ion Chiricuță” Oncological Institute Cluj Napoca

Revista Romana de Medicina de Laborator

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Objective: The aim of this research is to evaluate the value of the histological score based on a histological record compared to the histometry for monitoring...

Comparative assessment of bone regeneration by histometry and a histological scoring system / Evaluarea comparativă a regenerării osoase utilizând histometria și un scor de vindecare histologică

Published Online: Mar 25, 2015

Page range: 31 - 45

Received: Aug 14, 2014

Accepted: Mar 02, 2015

DOI: https://doi.org/10.1515/rrlm-2015-0009

Keywords
tissue engineering, histometry, bone healing, stem cells, histological scoring system

© by Ondine Lucaciu

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Comparative assessment of bone regeneration by histometry and a histological scoring system / Evaluarea comparativă a regenerării osoase utilizând histometria și un scor de vindecare histologică

Published Online: Mar 25, 2015

Page range: 31 - 45

Received: Aug 14, 2014

Accepted: Mar 02, 2015

DOI: https://doi.org/10.1515/rrlm-2015-0009

Keywordstissue engineering, histometry, bone healing, stem cells, histological scoring system

© by Ondine Lucaciu

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Keywords
tissue engineering, histometry, bone healing, stem cells, histological scoring system