<abstract xmlns="http://www.w3.org/1999/xhtml">The aim of this study was to determine the histological characteristics of the thyroid gland in ApoE KO-/- mice following the application of levothyroxine (l-thyroxine).A total of 12 female ApoE KO-/- mice were divided into two groups. A control group of mice received distilled water and the experimental group received l-thyroxine dissolved in drinking water at a daily dose of 2 µg/ml, over 12 weeks. The paraffin sections were processed with the usual haematoxilin-eosin technique of staining.Qualitative histological analysis demonstrated: the presence of large distended follicles in the peripheral areas of the gland; fulfillment of the lumen of follicles with an ample amount of colloid; complete absence of resorptive vacuoles in the colloid; a flattened follicullar epithelium. Morphometric assessment showed a significant increase in the diameters of follicles in the peripheral areas of the gland and a significant decrease in the height of the follicullar epithelium (p &lt;0,001).Our results demonstrated that l-thyroxine causes characteristic morphological changes in the structure of the thyroid gland in the direction of the occurrence of hyperthyroidism.</abstract>

The aim of this study was to determine the histological characteristics of the thyroid gland in ApoE KO-/- mice following the application of levothyroxine (l-thyroxine).A total of 12 female ApoE KO-/- mice were divided into two groups. A control group of mice received distilled water and the experimental group received l-thyroxine dissolved in drinking water at a daily dose of 2 µg/ml, over 12 weeks. The paraffin sections were processed with the usual haematoxilin-eosin technique of staining.Qualitative histological analysis demonstrated: the presence of large distended follicles in the peripheral areas of the gland; fulfillment of the lumen of follicles with an ample amount of colloid; complete absence of resorptive vacuoles in the colloid; a flattened follicullar epithelium. Morphometric assessment showed a significant increase in the diameters of follicles in the peripheral areas of the gland and a significant decrease in the height of the follicullar epithelium (p <0,001).Our results demonstrated that l-thyroxine causes characteristic morphological changes in the structure of the thyroid gland in the direction of the occurrence of hyperthyroidism.

Histological Structure of the Thyroid Gland in Apolipoprotein E Deficient Female Mice After Levothyroxine Application

Department of Histology and Embryology, Medical Faculty

PRILOZI

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

The aim of this study was to determine the histological characteristics of the thyroid gland in ApoE KO-/- mice following the application of levothyroxine...

Histological Structure of the Thyroid Gland in Apolipoprotein E Deficient Female Mice After Levothyroxine Application

Published Online: Mar 01, 2015

Page range: 135 - 140

DOI: https://doi.org/10.1515/prilozi-2015-0017

Keywords
thyroid gland, ApoE KO-/- female mice, morphometric analysis, l-thyroxine

© 2015 Irena Petrova et. al.

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Histological Structure of the Thyroid Gland in Apolipoprotein E Deficient Female Mice After Levothyroxine Application

Published Online: Mar 01, 2015

Page range: 135 - 140

DOI: https://doi.org/10.1515/prilozi-2015-0017

Keywordsthyroid gland, ApoE KO-/- female mice, morphometric analysis, l-thyroxine

© 2015 Irena Petrova et. al.

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Keywords
thyroid gland, ApoE KO-/- female mice, morphometric analysis, l-thyroxine