<abstract xmlns="http://www.w3.org/1999/xhtml"><p>Understanding a membrane’s morphology is important for controlling its final performance during protein immobilization. Porous, symmetric membranes were prepared from a polyvinylidene fluoride/N-methyl-2-pyrrolidinone solution by phase inversion process, to obtain membrane with various microsized pores. The concentration and surface area of aprotein dotted on the membrane surface were measured by staining with Ponceau S dye. The dotted protein was further scanned and analysed to perform quantitative measurements for relative comparison. The intensity of the red protein spot and its surface area varied depending on the membrane pore size, demonstrating the dependence of protein immobilization on this factor. The membrane with the smallest pore size (M3) showed the highest protein spot intensity and surface area when examined at different protein concentrations. An increase in the applied protein volume showed a linearity proportional trend to the total surface area, and an uneven round dot shape was observed at a large applied volume of protein solution.</p></abstract>

Understanding a membrane’s morphology is important for controlling its final performance during protein immobilization. Porous, symmetric membranes were prepared from a polyvinylidene fluoride/N-methyl-2-pyrrolidinone solution by phase inversion process, to obtain membrane with various microsized pores. The concentration and surface area of aprotein dotted on the membrane surface were measured by staining with Ponceau S dye. The dotted protein was further scanned and analysed to perform quantitative measurements for relative comparison. The intensity of the red protein spot and its surface area varied depending on the membrane pore size, demonstrating the dependence of protein immobilization on this factor. The membrane with the smallest pore size (M3) showed the highest protein spot intensity and surface area when examined at different protein concentrations. An increase in the applied protein volume showed a linearity proportional trend to the total surface area, and an uneven round dot shape was observed at a large applied volume of protein solution.

Impact of membrane pore structure on protein detection sensitivity of affinity-based immunoassay

Universiti Sains Malaysia, School of Chemical Engineering, Engineering Campus, Seri Ampangan, 14300 Nibong Tebal, S.P.S, Penang, Malaysia

Universiti Teknologi MARA, Faculty of Chemical Engineering, 40450 Shah Alam, Selangor, Malaysia

Universiti Sains Malaysia, Institute for Research in Molecular Medicine, Health Campus, 16150 Kubang Kerian, Kelantan, Malaysia

Polish Journal of Chemical Technology

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Understanding a membrane’s morphology is important for controlling its final performance during protein immobilization. Porous, symmetric membranes were...

Impact of membrane pore structure on protein detection sensitivity of affinity-based immunoassay

Published Online: Jun 30, 2016

Page range: 97 - 103

DOI: https://doi.org/10.1515/pjct-2016-0035

Keywords
morphology, PVDF membrane, protein immobilization

© 2016 A.L. Ahmad et al., published by De Gruyter Open

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Impact of membrane pore structure on protein detection sensitivity of affinity-based immunoassay

Published Online: Jun 30, 2016

Page range: 97 - 103

DOI: https://doi.org/10.1515/pjct-2016-0035

Keywordsmorphology, PVDF membrane, protein immobilization

© 2016 A.L. Ahmad et al., published by De Gruyter Open

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Keywords
morphology, PVDF membrane, protein immobilization