<abstract xmlns="http://www.w3.org/1999/xhtml"><p>The separation and purification of rebaudioside A from <italic>Stevia rebaudiana</italic> crude extracts (Steviosides) by macroporous resin were optimized by Taguchi orthogonal array (OA) experimental design methodology. This approach was applied to evaluate the influence of five factors (adsorption temperature, desorption time, elution solution ratio, adsorption volume and type of resin) on the rebaudioside A yield. The percentage contribution of each factor was also determined. The results showed that elution solution ratio and adsorption volume made the greatest (59.6%) and the lowest (1.3%) contribution, respectively. The results showed that the Taguchi method is able to model the purification of rebaudioside A process well (<italic>R</italic><sup>2</sup> > 0.998) and can therefore be applied in future studies conducted in various fields. Adsorption temperature 35°C, desorption time 60min, elution solution ratio 3, adsorption volume 200ml and HPD-400 as resin were the best conditions determined by the Taguchi method.</p></abstract>

The separation and purification of rebaudioside A from Stevia rebaudiana crude extracts (Steviosides) by macroporous resin were optimized by Taguchi orthogonal array (OA) experimental design methodology. This approach was applied to evaluate the influence of five factors (adsorption temperature, desorption time, elution solution ratio, adsorption volume and type of resin) on the rebaudioside A yield. The percentage contribution of each factor was also determined. The results showed that elution solution ratio and adsorption volume made the greatest (59.6%) and the lowest (1.3%) contribution, respectively. The results showed that the Taguchi method is able to model the purification of rebaudioside A process well (R2 > 0.998) and can therefore be applied in future studies conducted in various fields. Adsorption temperature 35°C, desorption time 60min, elution solution ratio 3, adsorption volume 200ml and HPD-400 as resin were the best conditions determined by the Taguchi method.

Separation and purification of rebaudioside A from extract of <italic>Stevia Rebaudiana</italic> leaves by macroporous adsorption resins

Islamic Azad University, Department of Biology, Faculty of Sciences, Rasht Branch, Rasht, Iran

University of Guilan, Department of Chemical Engineering, Technical Faculty, P.O. Box 41635-3756, Rasht, Iran

Polish Journal of Chemical Technology

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Separation and purification of rebaudioside A from extract of  leaves by macroporous adsorption resins

The separation and purification of rebaudioside A from Stevia rebaudiana crude extracts (Steviosides) by macroporous resin were optimized by Taguchi orthogonal...

Separation and purification of rebaudioside A from extract of Stevia Rebaudiana leaves by macroporous adsorption resins

Published Online: Apr 04, 2016

Page range: 127 - 132

DOI: https://doi.org/10.1515/pjct-2016-0019

Keywords
rebaudioside A, design of experimental, taguchi orthogonal array

© 2016 Masoumeh Anvari et al., published by De Gruyter Open

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Separation and purification of rebaudioside A from extract of Stevia Rebaudiana leaves by macroporous adsorption resins

Published Online: Apr 04, 2016

Page range: 127 - 132

DOI: https://doi.org/10.1515/pjct-2016-0019

Keywordsrebaudioside A, design of experimental, taguchi orthogonal array

© 2016 Masoumeh Anvari et al., published by De Gruyter Open

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Keywords
rebaudioside A, design of experimental, taguchi orthogonal array