1. bookVolume 52 (2015): Issue 2 (April 2015)
Journal Details
License
Format
Journal
eISSN
2255-8896
First Published
18 Mar 2008
Publication timeframe
6 times per year
Languages
English
access type Open Access

Influence of Light Intensity and Temperature on Cultivation of Microalgae Desmodesmus Communis in Flasks and Laboratory-Scale Stirred Tank Photobioreactor

Published Online: 22 May 2015
Volume & Issue: Volume 52 (2015) - Issue 2 (April 2015)
Page range: 59 - 70
Journal Details
License
Format
Journal
eISSN
2255-8896
First Published
18 Mar 2008
Publication timeframe
6 times per year
Languages
English
Abstract

Optimization of the microalgae cultivation process and of the bioprocess in general traditionally starts with cultivation experiments in flasks. Then the scale-up follows, when the process from flasks is transferred into a laboratory-scale bioreactor, in which further experiments are performed before developing the process in a pilot-scale reactor. This research was done in order to scale-up the process from a 0.4 1 shake flask to a 4.0 1 laboratory-scale stirred-tank photobioreactor for the cultivation of Desmodesmus (D.) communis microalgae. First, the effect of variation in temperature (21-29 ºC) and in light intensity (200-600 μmol m-2s-1) was studied in the shake-flask experiments. It was shown that the best results (the maximum biomass concentration of 2.72 g 1-1 with a specific growth rate of 0.65 g g-1d-1) can be achieved at the cultivation temperature and light intensity being 25 °C and 300 μmol m2s-1, respectively. At the same time, D. communis cultivation under the same conditions in stirred-tank photobioreactor resulted in average volumetric productivities of biomass due to the light limitation even when the light intensity was increased during the experiment (the maximum biomass productivity 0.25 g 1-1d-1; the maximum biomass concentration 1.78 g 1-1).

Keywords

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