<abstract xmlns="http://www.w3.org/1999/xhtml"><p> Introduction: The giant liver fluke, Fascioloides magna, has spread across Europe over the years posing a serious threat to the Polish cervid population. Material and Methods: Macroscopic and histopathological studies of the liver of 22 roe deer (Capreolus capreolus), 10 red deer (Cervus elaphus), and 6 fallow deer (Dama dama) were performed. Species determination of the recovered liver flukes and eggs was performed by PCR protocol amplifying fragments of ribosomal DNA (ITS2), according to a standard method. Results: The presence of F. magna was confirmed in three (13.6%) roe deer, seven (70.0%) red deer, and two (33.3%) fallow deer. The fluke eggs were found only in the stools of five red deer and one fallow deer. Conclusion: This study presents detailed pathological and histopathological changes in the liver of wild Polish cervids, including roe deer, which were subjected to such study for the first time. The hepatic lesions typical for different stages of liver cirrhosis varied depending on the host species and stage of the disease.</p></abstract>

Introduction: The giant liver fluke, Fascioloides magna, has spread across Europe over the years posing a serious threat to the Polish cervid population. Material and Methods: Macroscopic and histopathological studies of the liver of 22 roe deer (Capreolus capreolus), 10 red deer (Cervus elaphus), and 6 fallow deer (Dama dama) were performed. Species determination of the recovered liver flukes and eggs was performed by PCR protocol amplifying fragments of ribosomal DNA (ITS2), according to a standard method. Results: The presence of F. magna was confirmed in three (13.6%) roe deer, seven (70.0%) red deer, and two (33.3%) fallow deer. The fluke eggs were found only in the stools of five red deer and one fallow deer. Conclusion: This study presents detailed pathological and histopathological changes in the liver of wild Polish cervids, including roe deer, which were subjected to such study for the first time. The hepatic lesions typical for different stages of liver cirrhosis varied depending on the host species and stage of the disease.

Morphology and molecular study of Fascioloides magna – a growing threat to cervids (Cervidae) in Poland

Department of Pathology, Wroclaw University of Environmental and Life Sciences, 50-375 Wroclaw,

Department of Internal Medicine and Clinic of Diseases of Horses, Dogs, and Cats. Division of Parasitology, Wroclaw University of Environmental and Life Sciences, 50-375 Wroclaw,

Department of Food Hygiene and Consumer Health Protection, Faculty of Veterinary Medicine, Wroclaw University of Environmental and Life Sciences, 50-375 Wroclaw,

Journal of Veterinary Research

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 License.

Introduction: The giant liver fluke, Fascioloides magna, has spread across Europe over the years posing a serious threat to the Polish cervid population....

Morphology and molecular study of Fascioloides magna – a growing threat to cervids (Cervidae) in Poland

Published Online: Dec 17, 2016

Page range: 435 - 439

Received: Aug 02, 2016

Accepted: Nov 16, 2016

DOI: https://doi.org/10.1515/jvetres-2016-0065

Keywords
Cervidae, Fascioloides magna, liver, pathomorphology, PCR

© 2016

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 License.

Morphology and molecular study of Fascioloides magna – a growing threat to cervids (Cervidae) in Poland

Published Online: Dec 17, 2016

Page range: 435 - 439

Received: Aug 02, 2016

Accepted: Nov 16, 2016

DOI: https://doi.org/10.1515/jvetres-2016-0065

KeywordsCervidae, Fascioloides magna, liver, pathomorphology, PCR

© 2016

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 License.

Keywords
Cervidae, Fascioloides magna, liver, pathomorphology, PCR