<abstract xmlns="http://www.w3.org/1999/xhtml"><bold>Introduction:</bold> The present study aimed to investigate the effect of <italic>Enterococcus faecium</italic> EF55 on chickens, as well as its influence on proliferative activity of epithelial intestinal cells after infection with <italic>Salmonella enterica</italic> serovar Enteritidis phage type 4 (SE PT4). Moreover, the length and area of duodenal and jejunal villi of the birds were examined.<bold>Material and Methods:</bold> A pool of 80 birds was divided randomly into four groups. Probiotic group (EF) and Salmonella + probiotic group (EFSE) received <italic>E. faecium</italic> EF55 (109 CFU – 3 g per group/day) during 22 d. Salmonella group (SE) and EFSE group were infected with <italic>Salmonella</italic> Enteritidis PT4 (108 CFU in 0.2 mL PBS) in a single dose <italic>per os</italic> on day four of the experiment, whereas control birds (C group) received only 0.2 mL of PBS on that day. Samples were taken on the 4th and 18th day post infection.<bold>Results:</bold> Supplementation of feed with <italic>E. faecium</italic> EF 55 confirmed its selective antibacterial activity against SE PT4. The chickens infected with SE PT4 and fed <italic>E. faecium</italic> EF55 supplemented diet showed increased proliferative activity of enterocytes in the jejunum in both samplings. Applied probiotic strain demonstrated positive impact on intestinal morphometry in the jejunum of both non-infected groups and in <italic>Salmonella</italic>-infected chickens. In the latter group, the beneficial effect of <italic>E. faecium</italic> EF 55 was manifested by more efficient tissue turnover in the jejunum.</abstract>

Introduction: The present study aimed to investigate the effect of Enterococcus faecium EF55 on chickens, as well as its influence on proliferative activity of epithelial intestinal cells after infection with Salmonella enterica serovar Enteritidis phage type 4 (SE PT4). Moreover, the length and area of duodenal and jejunal villi of the birds were examined.Material and Methods: A pool of 80 birds was divided randomly into four groups. Probiotic group (EF) and Salmonella + probiotic group (EFSE) received E. faecium EF55 (109 CFU – 3 g per group/day) during 22 d. Salmonella group (SE) and EFSE group were infected with Salmonella Enteritidis PT4 (108 CFU in 0.2 mL PBS) in a single dose per os on day four of the experiment, whereas control birds (C group) received only 0.2 mL of PBS on that day. Samples were taken on the 4th and 18th day post infection.Results: Supplementation of feed with E. faecium EF 55 confirmed its selective antibacterial activity against SE PT4. The chickens infected with SE PT4 and fed E. faecium EF55 supplemented diet showed increased proliferative activity of enterocytes in the jejunum in both samplings. Applied probiotic strain demonstrated positive impact on intestinal morphometry in the jejunum of both non-infected groups and in Salmonella-infected chickens. In the latter group, the beneficial effect of E. faecium EF 55 was manifested by more efficient tissue turnover in the jejunum.

Effect of <italic>Enterococcus faecium</italic> EF 55 on morphometry and proliferative activity of intestinal mucosa in broilers infected with <italic>Salmonella</italic> Enteritidis

Journal of Veterinary Research

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Effect of  EF 55 on morphometry and proliferative activity of intestinal mucosa in broilers infected with  Enteritidis

Introduction: The present study aimed to investigate the effect of Enterococcus faecium EF55 on chickens, as well as its influence on proliferative activity of...

Effect of Enterococcus faecium EF 55 on morphometry and proliferative activity of intestinal mucosa in broilers infected with Salmonella Enteritidis

Published Online: Sep 23, 2016

Page range: 261 - 265

Received: Apr 07, 2016

Accepted: Sep 06, 2016

DOI: https://doi.org/10.1515/jvetres-2016-0040

Keywords
chickens, probiotic, intestinal morphometry, proliferative activity of enterocytes

© 2016 Zuzana Ševčíková et al., published by De Gruyter Open

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Effect of Enterococcus faecium EF 55 on morphometry and proliferative activity of intestinal mucosa in broilers infected with Salmonella Enteritidis

Published Online: Sep 23, 2016

Page range: 261 - 265

Received: Apr 07, 2016

Accepted: Sep 06, 2016

DOI: https://doi.org/10.1515/jvetres-2016-0040

Keywordschickens, probiotic, intestinal morphometry, proliferative activity of enterocytes

© 2016 Zuzana Ševčíková et al., published by De Gruyter Open

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Keywords
chickens, probiotic, intestinal morphometry, proliferative activity of enterocytes