<abstract xmlns="http://www.w3.org/1999/xhtml"><bold>Introduction:</bold> In the present study apoptosis was investigated in the cornu ammonis and cerebellum of 10 dogs naturally infected with rabies virus. Diagnosis of rabies was based on the results of fluorescent antibody staining and experimental inoculation.<bold>Material and Methods:</bold> The paraffin tissue sections were stained with haematoxylin and eosin, avidin-biotin complex peroxidase (ABC-P), and terminal deoxynucleotidyl transferase biotin-dUTP nick end-labelling (TUNEL) methods.<bold>Results:</bold> Histopathological examination revealed encephalomyelitis of varying severity and the presence of Negri bodies. Dense rabies antigens were determined in the motor neurons with ABC-P method. On the other hand, Bcl-2 protein and Bax protein gave positive reaction in seven and five cases, respectively. TUNEL staining demonstrated very marked apoptotic changes in the nuclei of neurons localised deep in the substantia alba of the cerebellum. Similar changes were also determined in perivascular mononuclear cells and glia cells within the substantia alba. No apoptopic changes were found in the motor neurons of the cornu ammonis.<bold>Conclusion:</bold> The absence of apoptotic changes in the neurons was considered to be the consequence of the necrotic changes that developed in these neurons.</abstract>

Introduction: In the present study apoptosis was investigated in the cornu ammonis and cerebellum of 10 dogs naturally infected with rabies virus. Diagnosis of rabies was based on the results of fluorescent antibody staining and experimental inoculation.Material and Methods: The paraffin tissue sections were stained with haematoxylin and eosin, avidin-biotin complex peroxidase (ABC-P), and terminal deoxynucleotidyl transferase biotin-dUTP nick end-labelling (TUNEL) methods.Results: Histopathological examination revealed encephalomyelitis of varying severity and the presence of Negri bodies. Dense rabies antigens were determined in the motor neurons with ABC-P method. On the other hand, Bcl-2 protein and Bax protein gave positive reaction in seven and five cases, respectively. TUNEL staining demonstrated very marked apoptotic changes in the nuclei of neurons localised deep in the substantia alba of the cerebellum. Similar changes were also determined in perivascular mononuclear cells and glia cells within the substantia alba. No apoptopic changes were found in the motor neurons of the cornu ammonis.Conclusion: The absence of apoptotic changes in the neurons was considered to be the consequence of the necrotic changes that developed in these neurons.

Apoptosis in natural rabies virus infection in dogs

Department of Pathology, Faculty of Veterinary Medicine, Ankara University, Diskapi, 06110, Ankara,

Department of Pathology, Faculty of Veterinary Medicine, Afyon Kocatepe University, 03200, Afyonkarahisar,

Pendik Veterinary Control and Research Institute, Pendik, 34890, Istanbul,

Department of Pathology, Faculty of Veterinary Medicine, Balıkesir University, 10145, Balıkesir,

Journal of Veterinary Research

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

{"article-title":"Apoptosis in natural rabies virus infection in dogs"}

Introduction: In the present study apoptosis was investigated in the cornu ammonis and cerebellum of 10 dogs naturally infected with rabies virus. Diagnosis of...

Apoptosis in natural rabies virus infection in dogs

Published Online: Sep 23, 2016

Page range: 227 - 231

Received: Oct 30, 2015

Accepted: Aug 29, 2016

DOI: https://doi.org/10.1515/jvetres-2016-0034

Keywords
dogs, rabies, central nervous system, apoptosis, histopathology

© 2016 Sevil Atalay Vural et al., published by De Gruyter Open

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Apoptosis in natural rabies virus infection in dogs

Published Online: Sep 23, 2016

Page range: 227 - 231

Received: Oct 30, 2015

Accepted: Aug 29, 2016

DOI: https://doi.org/10.1515/jvetres-2016-0034

Keywordsdogs, rabies, central nervous system, apoptosis, histopathology

© 2016 Sevil Atalay Vural et al., published by De Gruyter Open

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Keywords
dogs, rabies, central nervous system, apoptosis, histopathology