<abstract xmlns="http://www.w3.org/1999/xhtml"><p> Introduction: The article presents a rapid and simple analytical procedure for determination of four tetracyclines (oxytetracycline, tetracycline, chlortetracycline, and doxycycline) in animal medicated feedingstuffs. Material and Methods: Two-gramme samples were extracted by a Na<sub>2</sub>EDTA-McIlvaine buffer (pH 4)/methanol mixtures (40/60, v/v). The determination was achieved by liquid chromatography using a Zorbax Eclipse XDB C18 analytical column with mass spectrometer detection (LC-MS). Results: Recoveries of the antibiotics from spiked feed samples ranged from 78.2% to 113.5%. The LOD and LOQ for tetracyclines in feeds ranged from 2.8 to 4.2 and from 4.3 to 5.7 mg/kg, respectively. Conclusion: The method was successfully validated and proved to be efficient, precise, and useful for quantification of tetracyclines in medicated feedingstuffs.</p></abstract>

Introduction: The article presents a rapid and simple analytical procedure for determination of four tetracyclines (oxytetracycline, tetracycline, chlortetracycline, and doxycycline) in animal medicated feedingstuffs. Material and Methods: Two-gramme samples were extracted by a Na2EDTA-McIlvaine buffer (pH 4)/methanol mixtures (40/60, v/v). The determination was achieved by liquid chromatography using a Zorbax Eclipse XDB C18 analytical column with mass spectrometer detection (LC-MS). Results: Recoveries of the antibiotics from spiked feed samples ranged from 78.2% to 113.5%. The LOD and LOQ for tetracyclines in feeds ranged from 2.8 to 4.2 and from 4.3 to 5.7 mg/kg, respectively. Conclusion: The method was successfully validated and proved to be efficient, precise, and useful for quantification of tetracyclines in medicated feedingstuffs.

Analytical procedure for the determination of tetracyclines in medicated feedingstuffs by liquid chromatography-mass spectrometry

Department of Hygiene of Animal Feedingstuffs National Veterinary Research Institute, 24-100 Pulawy, Poland

Journal of Veterinary Research

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 License.

Introduction: The article presents a rapid and simple analytical procedure for determination of four tetracyclines (oxytetracycline, tetracycline,...

Analytical procedure for the determination of tetracyclines in medicated feedingstuffs by liquid chromatography-mass spectrometry

Published Online: Apr 29, 2016

Page range: 35 - 41

Received: Oct 26, 2015

Accepted: Mar 10, 2016

DOI: https://doi.org/10.1515/jvetres-2016-0006

Keywords
feeds, tetracyclines, LC-MS, solid-liquid extraction

© 2016

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 License.

Analytical procedure for the determination of tetracyclines in medicated feedingstuffs by liquid chromatography-mass spectrometry

Published Online: Apr 29, 2016

Page range: 35 - 41

Received: Oct 26, 2015

Accepted: Mar 10, 2016

DOI: https://doi.org/10.1515/jvetres-2016-0006

Keywordsfeeds, tetracyclines, LC-MS, solid-liquid extraction

© 2016

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 License.

Keywords
feeds, tetracyclines, LC-MS, solid-liquid extraction