<abstract xmlns="http://www.w3.org/1999/xhtml"><p> Introduction: The aim of this study was to use TaqMan real-time PCR technique to investigate Jaagsiekte sheep retrovirus (JSRV) proviral DNA in whole blood samples of sheep, and compare the results to those of histopathological examinations. Material and Methods: Eighty blood samples from clinically healthy sheep were randomly collected before the animals were slaughtered. Ten tissue samples from each lung and associated caudal mediastinal lymph node were taken. Results: Fifteen (18.75%) blood samples were found to contain proviral DNA, and 11 (13.75%) corresponding lung samples showed microscopic changes consistent with ovine pulmonary adenocarcinoma. None of the samples displayed metastases to the caudal mediastinal lymph nodes. The prominent pattern of neoplastic nodules consisted of acinar (alveolar) form. Conclusion: The results indicated the higher sensitivity of real-time PCR compared to histopathological examinations in detection of ovine pulmonary adenocarcinoma.</p></abstract>

Introduction: The aim of this study was to use TaqMan real-time PCR technique to investigate Jaagsiekte sheep retrovirus (JSRV) proviral DNA in whole blood samples of sheep, and compare the results to those of histopathological examinations. Material and Methods: Eighty blood samples from clinically healthy sheep were randomly collected before the animals were slaughtered. Ten tissue samples from each lung and associated caudal mediastinal lymph node were taken. Results: Fifteen (18.75%) blood samples were found to contain proviral DNA, and 11 (13.75%) corresponding lung samples showed microscopic changes consistent with ovine pulmonary adenocarcinoma. None of the samples displayed metastases to the caudal mediastinal lymph nodes. The prominent pattern of neoplastic nodules consisted of acinar (alveolar) form. Conclusion: The results indicated the higher sensitivity of real-time PCR compared to histopathological examinations in detection of ovine pulmonary adenocarcinoma.

Detection of Jaagsiekte sheep retrovirus in apparently healthy sheep by real-time TaqMan PCR in comparison with histopathological findings

Department of Clinical Sciences, Faculty of Veterinary Science, Bu-Ali Sina University, 65176-58978 Hamedan, Iran

Department of Microbiology, Faculty of Medicine, Hamedan University of Medical Sciences, 65176-19654 Hamedan, Iran

Department of Pathobiology, Faculty of Veterinary Medicine, Lorestan University, 68137-17133 Khoramabad, Iran

Department of Clinical Sciences, Faculty of Veterinary Medicine, Tabriz University, 51666-16471 Tabriz, Iran

Journal of Veterinary Research

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 License.

Introduction: The aim of this study was to use TaqMan real-time PCR technique to investigate Jaagsiekte sheep retrovirus (JSRV) proviral DNA in whole blood...

Detection of Jaagsiekte sheep retrovirus in apparently healthy sheep by real-time TaqMan PCR in comparison with histopathological findings

Published Online: Apr 29, 2016

Page range: 7 - 12

Received: Nov 03, 2015

Accepted: Feb 15, 2016

DOI: https://doi.org/10.1515/jvetres-2016-0002

Keywords
sheep, ovine pulmonary adenocarcinoma, Jaagsiekte retrovirus, real-time PCR, histopathology

© 2016

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 License.

Detection of Jaagsiekte sheep retrovirus in apparently healthy sheep by real-time TaqMan PCR in comparison with histopathological findings

Published Online: Apr 29, 2016

Page range: 7 - 12

Received: Nov 03, 2015

Accepted: Feb 15, 2016

DOI: https://doi.org/10.1515/jvetres-2016-0002

Keywordssheep, ovine pulmonary adenocarcinoma, Jaagsiekte retrovirus, real-time PCR, histopathology

© 2016

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 License.

Keywords
sheep, ovine pulmonary adenocarcinoma, Jaagsiekte retrovirus, real-time PCR, histopathology