<abstract xmlns="http://www.w3.org/1999/xhtml"> Transverse sections (2 mm thickness) of stem-nodes from in vitro raised seedlings had morphogenic potential on semisolid and liquid Murashige and Skoog medium supplemented with cytokinins N6-benzyladenine (BA 4.44 μM), furfurylaminopurine (KIN 4.65 μM) and auxin α-naphthalene acetic acid (NAA 5.37 μM) individually and in combinations. The regeneration response was influenced by both the type of growth regulator and physical state of the medium. The explants produced either shoot buds on cytokinincontaining media or protocorm-like bodies (PLBs) on NAA containing media both solid and liquid. More neo-formations were produced on liquid media, especially those containing only NAA. They were formed at nodal and inter-nodal regions. The secondary buds were produced on the surface of primary PLBs. The plantlets were developed on MS medium containing banana homogenate 50 g·dm-3. The current study is the first ever report on successful regeneration of Dendrobium chrysotoxum from stem-node segments.</abstract>

Transverse sections (2 mm thickness) of stem-nodes from in vitro raised seedlings had morphogenic potential on semisolid and liquid Murashige and Skoog medium supplemented with cytokinins N6-benzyladenine (BA 4.44 μM), furfurylaminopurine (KIN 4.65 μM) and auxin α-naphthalene acetic acid (NAA 5.37 μM) individually and in combinations. The regeneration response was influenced by both the type of growth regulator and physical state of the medium. The explants produced either shoot buds on cytokinincontaining media or protocorm-like bodies (PLBs) on NAA containing media both solid and liquid. More neo-formations were produced on liquid media, especially those containing only NAA. They were formed at nodal and inter-nodal regions. The secondary buds were produced on the surface of primary PLBs. The plantlets were developed on MS medium containing banana homogenate 50 g·dm-3. The current study is the first ever report on successful regeneration of Dendrobium chrysotoxum from stem-node segments.

In Vitro Regeneration of Shoots From Nodal Explants of Dendrobium Chrysotoxum Lindl

Journal of Horticultural Research

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 License.

Transverse sections (2 mm thickness) of stem-nodes from in vitro raised seedlings had morphogenic potential on semisolid and liquid Murashige and Skoog medium...

In Vitro Regeneration of Shoots From Nodal Explants of Dendrobium Chrysotoxum Lindl

Published Online: Jun 30, 2017

Page range: 27 - 34

Received: Mar 01, 2017

Accepted: Jun 01, 2017

DOI: https://doi.org/10.1515/johr-2017-0003

Keywords
Dendrobium chrysotoxum, endangered species, species preservation, protocorm-like body, nodal explants

© by Saranjeet Kaur

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 License.

In Vitro Regeneration of Shoots From Nodal Explants of Dendrobium Chrysotoxum Lindl

Published Online: Jun 30, 2017

Page range: 27 - 34

Received: Mar 01, 2017

Accepted: Jun 01, 2017

DOI: https://doi.org/10.1515/johr-2017-0003

KeywordsDendrobium chrysotoxum, endangered species, species preservation, protocorm-like body, nodal explants

© by Saranjeet Kaur

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 License.

Keywords
Dendrobium chrysotoxum, endangered species, species preservation, protocorm-like body, nodal explants