<abstract xmlns="http://www.w3.org/1999/xhtml"><p><italic>Clerodendrum phlomidis</italic> L. f. is an important medicinal plant of the Lamiaceae family, particularly its roots, which are used for various therapeutic purposes in a pulverized form. The objective of this study was to develop a standard protocol for axillary shoot proliferation and rooting of <italic>C. phlomidis</italic> for its propagation and conservation. Nodal explants were inoculated on Murashige and Skoog (MS) medium that was supplemented with one of six cytokinins: 6-benzyladenine, kinetin, thidiazuron, <italic>N</italic><sup>6</sup>-(2-isopentenyl) adenine (2iP), <italic>trans</italic>-zeatin (Zea) and <italic>meta</italic>-topolin. Callus induction, which was prolific at all concentrations, formed at the base of nodal explants and hindered shoot multiplication and elongation. To avoid or reduce callus formation with the objective of increasing shoot formation, the same six cytokinins were combined with 4 μM 2,3,5-tri-iodobenzoic acid (TIBA) alone or in combination with 270 μM adenine sulphate (AdS). Nodal explants that were cultured on the medium supplemented with 9.12 μM Zea, 4 μM TIBA and 270 μM AdS produced significantly more and longer shoots than on medium without TIBA and AdS. Half-strength MS medium supplemented with 8.05 μM α-naphthaleneacetic acid was the best medium for root formation. Most (75%) <italic>in vitro</italic> rooted plantlets were successfully acclimatized under natural conditions.</p></abstract>

Clerodendrum phlomidis L. f. is an important medicinal plant of the Lamiaceae family, particularly its roots, which are used for various therapeutic purposes in a pulverized form. The objective of this study was to develop a standard protocol for axillary shoot proliferation and rooting of C. phlomidis for its propagation and conservation. Nodal explants were inoculated on Murashige and Skoog (MS) medium that was supplemented with one of six cytokinins: 6-benzyladenine, kinetin, thidiazuron, N6-(2-isopentenyl) adenine (2iP), trans-zeatin (Zea) and meta-topolin. Callus induction, which was prolific at all concentrations, formed at the base of nodal explants and hindered shoot multiplication and elongation. To avoid or reduce callus formation with the objective of increasing shoot formation, the same six cytokinins were combined with 4 μM 2,3,5-tri-iodobenzoic acid (TIBA) alone or in combination with 270 μM adenine sulphate (AdS). Nodal explants that were cultured on the medium supplemented with 9.12 μM Zea, 4 μM TIBA and 270 μM AdS produced significantly more and longer shoots than on medium without TIBA and AdS. Half-strength MS medium supplemented with 8.05 μM α-naphthaleneacetic acid was the best medium for root formation. Most (75%) in vitro rooted plantlets were successfully acclimatized under natural conditions.

Micropropagation of <italic>C<sc>lerodendrum phlomidis</sc></italic> L.F.

B.R. Doshi School of Biosciences, Sardar Patel University, Sardar Patel Maidan, Vadtal Road, Satellite Campus, Post Box No. 39, Vallabh Vidyanagar-388120, India

P.O. Box 7, Miki-cho post office 3011-2, Ikenobe, Kagawa-ken 761-0799, Japan

Journal of Horticultural Research

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Clerodendrum phlomidis L. f. is an important medicinal plant of the Lamiaceae family, particularly its roots, which are used for various therapeutic purposes...

Micropropagation of Clerodendrum phlomidis L.F.

Published Online: Jul 27, 2016

Page range: 21 - 28

Received: Mar 01, 2016

Accepted: May 01, 2016

DOI: https://doi.org/10.1515/johr-2016-0003

Keywords
auxin inhibitor, axillary shoot multiplication, callus, nodal explants, 2,3,5-tri-iodobenzoic acid

© 2016 Mafatlal M. Kher et al., published by De Gruyter Open

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Micropropagation of Clerodendrum phlomidis L.F.

Published Online: Jul 27, 2016

Page range: 21 - 28

Received: Mar 01, 2016

Accepted: May 01, 2016

DOI: https://doi.org/10.1515/johr-2016-0003

Keywordsauxin inhibitor, axillary shoot multiplication, callus, nodal explants, 2,3,5-tri-iodobenzoic acid

© 2016 Mafatlal M. Kher et al., published by De Gruyter Open

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Keywords
auxin inhibitor, axillary shoot multiplication, callus, nodal explants, 2,3,5-tri-iodobenzoic acid