<abstract xmlns="http://www.w3.org/1999/xhtml"><p>The enzymatic method for the determination of serum creatinine is accepted as one of the most accurate methods in a clinical laboratory. This method was used on a biochemistry auto analyzer (Cobas Integra 400) to determine serum creatinine at the laboratory of University Hospital – Pleven. The characteristics and reliability of this enzymatic method for creatinine were compared with the Jaffe kinetic method. Effects of some interfering substances like bilirubin and glucose on the Jaffe kinetic method and the enzymatic method were compared. Glucose and bilirubin inhibit the reaction between creatinine and alkaline picrate. Glucose slowly reduces picric acid to picrate, while the bilirubin present in a sample is oxidized to biliverdin under alkaline conditions. This leads to a decrease in absorbance at 520 nm. We measured creatinine in serum samples with the enzymatic method and the Jaffe kinetic method in samples divided into four groups: group I –samples without bilirubin and glucose ; group II –samples with high level of glucose; group III - samples with high level of bilirubin, group IV – all samples. For Group I, the correlation coefficient obtained by comparing the enzymatic creatinine method and Jaffe's kinetic method was R = 0.983. There was a very good agreement between the two methods in terms of correlation coefficient even in the samples with high levels of glucose or bilirubin.</p></abstract>

The enzymatic method for the determination of serum creatinine is accepted as one of the most accurate methods in a clinical laboratory. This method was used on a biochemistry auto analyzer (Cobas Integra 400) to determine serum creatinine at the laboratory of University Hospital – Pleven. The characteristics and reliability of this enzymatic method for creatinine were compared with the Jaffe kinetic method. Effects of some interfering substances like bilirubin and glucose on the Jaffe kinetic method and the enzymatic method were compared. Glucose and bilirubin inhibit the reaction between creatinine and alkaline picrate. Glucose slowly reduces picric acid to picrate, while the bilirubin present in a sample is oxidized to biliverdin under alkaline conditions. This leads to a decrease in absorbance at 520 nm. We measured creatinine in serum samples with the enzymatic method and the Jaffe kinetic method in samples divided into four groups: group I –samples without bilirubin and glucose ; group II –samples with high level of glucose; group III - samples with high level of bilirubin, group IV – all samples. For Group I, the correlation coefficient obtained by comparing the enzymatic creatinine method and Jaffe's kinetic method was R = 0.983. There was a very good agreement between the two methods in terms of correlation coefficient even in the samples with high levels of glucose or bilirubin.

Effects Of Glucose And Bilirubin On The Kinetic Jaffe's And The Enzymatic Methods For Serum Creatinine Assay

Clinical Laboratory, Medical University – Pleven

Journal of Biomedical and Clinical Research

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

The enzymatic method for the determination of serum creatinine is accepted as one of the most accurate methods in a clinical laboratory. This method was used...

Effects Of Glucose And Bilirubin On The Kinetic Jaffe's And The Enzymatic Methods For Serum Creatinine Assay

Published Online: Dec 10, 2015

Page range: 35 - 39

Received: Mar 30, 2015

Accepted: Apr 03, 2015

DOI: https://doi.org/10.1515/jbcr-2015-0149

Keywords
creatinine, enzymatic assay, Jaffe kinetic method, glucose, bilirubin

© 2015 Irena I. Gencheva et al., published by De Gruyter Open

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Effects Of Glucose And Bilirubin On The Kinetic Jaffe's And The Enzymatic Methods For Serum Creatinine Assay

Published Online: Dec 10, 2015

Page range: 35 - 39

Received: Mar 30, 2015

Accepted: Apr 03, 2015

DOI: https://doi.org/10.1515/jbcr-2015-0149

Keywordscreatinine, enzymatic assay, Jaffe kinetic method, glucose, bilirubin

© 2015 Irena I. Gencheva et al., published by De Gruyter Open

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Keywords
creatinine, enzymatic assay, Jaffe kinetic method, glucose, bilirubin