<abstract xmlns="http://www.w3.org/1999/xhtml"><p> A method was elaborated to determine phenolic compounds (vanillin, caffeic, p-coumaric and salicylic acids, and flavonoids: rutin, hesperetin, quercetin, pinocembrin, apigenin, kaempferol, isorhamnetin, chrysin, and acacetin) in bee pollen loads using highperformance liquid chromatography with a diode array detector (HPLC-DAD). Phenolic compounds from bee pollen were isolated on Cleanert C18-SPE columns (500 mg/6 mL, Agela Technologies). Polyphenols were identified by comparing the retention times and spectra of compounds found in pollen load samples with the ones of the standard mixture. Quantitative analysis was conducted using the external standard method. In addition, basic validation parameters for the method were determined. For the identified compounds (except for the salicylic acid), satisfactory (≥0.997) linear correlations were obtained. The elaborated method showed high repeatability and inter-laboratory reproducibility. Variability coeffcients of the majority of phenolic compounds did not exceed 10% in conditions of repeatability and inter-laboratory reproducibility, and for the total polyphenolic content they were 1.7 and 5.1%, respectively. The pollen load samples (n = 15) differed in qualitative and quantitative composition of the phenolic compounds. In all the samples, we identified the p-coumaric and salicylic acids and flavonoids rutin, hesperetin, and apigenin nevertheless, these compounds’ contents significantly differed among individual samples. The total phenolic content in the tested samples of pollen loads ranged from 0.653 to 5.966 mg/100 g (on average 2.737 mg/100 g).</p></abstract>

A method was elaborated to determine phenolic compounds (vanillin, caffeic, p-coumaric and salicylic acids, and flavonoids: rutin, hesperetin, quercetin, pinocembrin, apigenin, kaempferol, isorhamnetin, chrysin, and acacetin) in bee pollen loads using highperformance liquid chromatography with a diode array detector (HPLC-DAD). Phenolic compounds from bee pollen were isolated on Cleanert C18-SPE columns (500 mg/6 mL, Agela Technologies). Polyphenols were identified by comparing the retention times and spectra of compounds found in pollen load samples with the ones of the standard mixture. Quantitative analysis was conducted using the external standard method. In addition, basic validation parameters for the method were determined. For the identified compounds (except for the salicylic acid), satisfactory (≥0.997) linear correlations were obtained. The elaborated method showed high repeatability and inter-laboratory reproducibility. Variability coeffcients of the majority of phenolic compounds did not exceed 10% in conditions of repeatability and inter-laboratory reproducibility, and for the total polyphenolic content they were 1.7 and 5.1%, respectively. The pollen load samples (n = 15) differed in qualitative and quantitative composition of the phenolic compounds. In all the samples, we identified the p-coumaric and salicylic acids and flavonoids rutin, hesperetin, and apigenin nevertheless, these compounds’ contents significantly differed among individual samples. The total phenolic content in the tested samples of pollen loads ranged from 0.653 to 5.966 mg/100 g (on average 2.737 mg/100 g).

Application of HPLC-DAD Technique for Determination of Phenolic Compounds in Bee Pollen Loads

Journal of Apicultural Science

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 License.

A method was elaborated to determine phenolic compounds (vanillin, caffeic, p-coumaric and salicylic acids, and flavonoids: rutin, hesperetin, quercetin,...

Application of HPLC-DAD Technique for Determination of Phenolic Compounds in Bee Pollen Loads

Article Category: Short Communication

Published Online: Jun 16, 2017

Page range: 153 - 162

Received: Dec 22, 2016

Accepted: Apr 29, 2016

DOI: https://doi.org/10.1515/jas-2017-0009

Keywords
bee pollen, flavonoids, phenols, phenolic acid, HPLC-DAD

© by Ewa Waś

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 License.

Application of HPLC-DAD Technique for Determination of Phenolic Compounds in Bee Pollen Loads

Article Category: Short Communication

Published Online: Jun 16, 2017

Page range: 153 - 162

Received: Dec 22, 2016

Accepted: Apr 29, 2016

DOI: https://doi.org/10.1515/jas-2017-0009

Keywordsbee pollen, flavonoids, phenols, phenolic acid, HPLC-DAD

© by Ewa Waś

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 License.

Keywords
bee pollen, flavonoids, phenols, phenolic acid, HPLC-DAD