<abstract xmlns="http://www.w3.org/1999/xhtml"><p>The colony collapse disorder is a growing problem world-wide. For this reason, we were prompted to search for natural and harmless agents that could improve the living conditions of honey bees. This group of agents includes exogenous antioxidants, such as ascorbic acid, which boost natural immunity. We analysed the effect of vitamin C supplementation on carbohydrate metabolism in the developing honey bee worker brood. The total carbohydrate content and the concentrations of glycogen, trehalose, maltotriose, fructose, and glucose were estimated. The correlations between sugar content and the activity of the main hydrolases of carbohydrate metabolism - α-amylase, glucoamylase, trehalase, maltase, and sucrase - were determined. The addition of vitamin C to the diet of wintering bees did not impair their sugar metabolism. Vitamin C supplements exerted a positive effect by significantly increasing glycogen and trehalose concentrations in the initial phase of development and in newly emerged workers. Vitamin C did not induce significant changes in the developmental profile of carbohydrate degrading enzymes, except for the earliest stage of larval development when enzyme activity levels were below those noted in the control group.</p></abstract>

The colony collapse disorder is a growing problem world-wide. For this reason, we were prompted to search for natural and harmless agents that could improve the living conditions of honey bees. This group of agents includes exogenous antioxidants, such as ascorbic acid, which boost natural immunity. We analysed the effect of vitamin C supplementation on carbohydrate metabolism in the developing honey bee worker brood. The total carbohydrate content and the concentrations of glycogen, trehalose, maltotriose, fructose, and glucose were estimated. The correlations between sugar content and the activity of the main hydrolases of carbohydrate metabolism - α-amylase, glucoamylase, trehalase, maltase, and sucrase - were determined. The addition of vitamin C to the diet of wintering bees did not impair their sugar metabolism. Vitamin C supplements exerted a positive effect by significantly increasing glycogen and trehalose concentrations in the initial phase of development and in newly emerged workers. Vitamin C did not induce significant changes in the developmental profile of carbohydrate degrading enzymes, except for the earliest stage of larval development when enzyme activity levels were below those noted in the control group.

The Effect of Dietary Vitamin C on Carbohydrate Concentrations and Hydrolase Activity, During the Development of Honey Bee Worker Brood

University of Warmia and Mazury, Faculty of Biology and Biotechnology, Department of Biochemistry, Oczapowski 1A, 10-957 Olsztyn, Poland

Institute of Animal Reproduction and Food Research, The Polish Academy of Sciences, Bydgoska 1/8, 10-243 Olsztyn, Poland

Journal of Apicultural Science

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

The colony collapse disorder is a growing problem world-wide. For this reason, we were prompted to search for natural and harmless agents that could improve...

The Effect of Dietary Vitamin C on Carbohydrate Concentrations and Hydrolase Activity, During the Development of Honey Bee Worker Brood

Published Online: May 29, 2015

Page range: 5 - 16

Received: Nov 22, 2013

Accepted: Dec 18, 2014

DOI: https://doi.org/10.1515/jas-2015-0001

© by Małgorzata Dmitryjuk

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.