<abstract xmlns="http://www.w3.org/1999/xhtml"><p><italic>Aphelenchoides fragariae</italic> (Ritzema Bos, 1890) Christie, 1932 was isolated from leaves of silver birch (<italic>Betula pendula</italic> Roth) seedlings proving that the source of infection was anemones plants. This is the first report to our best knowledge showing that the source of nematode infection of a woody plant could be a perennial plant. <italic>A. fragariae</italic> was identified by morphometric and molecular analyses. Morphological diagnosis based on the bending shape of the tail of males and pronounced apex and rostrum proved to be the most accurate reliable characteristic. On the opposite, the high variability of the mucron shape in female tails made the identification by microscopic analyses difficult. Identification of the species was confirmed by analysis of 28S rDNA sequences. The morphometric data of adults extracted from silver birch was compared with that of nematodes isolated from <italic>Anemone hupehensis</italic> (Lemoine) Lemoine. Males body length varied highly in samples collected from both host plant species.</p></abstract>

Aphelenchoides fragariae (Ritzema Bos, 1890) Christie, 1932 was isolated from leaves of silver birch (Betula pendula Roth) seedlings proving that the source of infection was anemones plants. This is the first report to our best knowledge showing that the source of nematode infection of a woody plant could be a perennial plant. A. fragariae was identified by morphometric and molecular analyses. Morphological diagnosis based on the bending shape of the tail of males and pronounced apex and rostrum proved to be the most accurate reliable characteristic. On the opposite, the high variability of the mucron shape in female tails made the identification by microscopic analyses difficult. Identification of the species was confirmed by analysis of 28S rDNA sequences. The morphometric data of adults extracted from silver birch was compared with that of nematodes isolated from Anemone hupehensis (Lemoine) Lemoine. Males body length varied highly in samples collected from both host plant species.

Morphological and molecular characteristics of foliar nematode attacking silver birch (<italic>Betula pendula</italic> Roth) in Poland

Helminthologia

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Morphological and molecular characteristics of foliar nematode attacking silver birch ( Roth) in Poland

Aphelenchoides fragariae (Ritzema Bos, 1890) Christie, 1932 was isolated from leaves of silver birch (Betula pendula Roth) seedlings proving that the source of...

Morphological and molecular characteristics of foliar nematode attacking silver birch (Betula pendula Roth) in Poland

Published Online: Aug 02, 2017

Page range: 250 - 256

Received: Nov 09, 2015

Accepted: May 02, 2017

DOI: https://doi.org/10.1515/helm-2017-0032

Keywords
woody plant, morphometric data, 28S rDNA

© 2017 A. Chałańska et al., published by De Gruyter Open

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Morphological and molecular characteristics of foliar nematode attacking silver birch (Betula pendula Roth) in Poland

Published Online: Aug 02, 2017

Page range: 250 - 256

Received: Nov 09, 2015

Accepted: May 02, 2017

DOI: https://doi.org/10.1515/helm-2017-0032

Keywordswoody plant, morphometric data, 28S rDNA

© 2017 A. Chałańska et al., published by De Gruyter Open

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Keywords
woody plant, morphometric data, 28S rDNA