<abstract xmlns="http://www.w3.org/1999/xhtml">An investigation was conducted to study the effects of explant sources, plant growth regulators, carbohydrates and light conditions on indirect cormlet regeneration and the induction of embryogenic callus of freesia (<italic>Freesia</italic> × <italic>hybrida</italic> Bailey ‘Argenta’). Sections of two different types of explants, corms and pupae (cold storage-produced corms), were placed on Murashige and Skoog (MS) media containing different concentrations of plant growth regulators. The results showed that the highest percentage of callus induction (100%), the highest callus growth (15 mm diameter) and the best type of calli were achieved for pupa explants grown on the medium that contained 4 mg L−1 1-naphthaleneacetic acid (NAA) and 2 mg L−1 6-benzylaminopurine (BAP) in the dark. Increasing BAP up to 3 to 4.5 mg L−1 resulted in the maximum number of regenerated cormlets from 1 cm2 calli (2 cormlets) under light conditions. Overall, the best rooting of regenerated cormlets was achieved on MS media supplemented with 1 mg L−1 indole-3-butyric acid (IBA). In the next stage, high quality calli were subcultured on MS media containing sorbitol, sucrose, maltose and mannitol (0, 5, 10 and 15 g L−1). The results indicated that 15 g L−1 maltose was able to induce the highest percentage of embryogenic callus, with an average of 88.9% on media containing 2 mg L−1 BAP and 1 mg L−1 NAA.</abstract>

An investigation was conducted to study the effects of explant sources, plant growth regulators, carbohydrates and light conditions on indirect cormlet regeneration and the induction of embryogenic callus of freesia (Freesia × hybrida Bailey ‘Argenta’). Sections of two different types of explants, corms and pupae (cold storage-produced corms), were placed on Murashige and Skoog (MS) media containing different concentrations of plant growth regulators. The results showed that the highest percentage of callus induction (100%), the highest callus growth (15 mm diameter) and the best type of calli were achieved for pupa explants grown on the medium that contained 4 mg L−1 1-naphthaleneacetic acid (NAA) and 2 mg L−1 6-benzylaminopurine (BAP) in the dark. Increasing BAP up to 3 to 4.5 mg L−1 resulted in the maximum number of regenerated cormlets from 1 cm2 calli (2 cormlets) under light conditions. Overall, the best rooting of regenerated cormlets was achieved on MS media supplemented with 1 mg L−1 indole-3-butyric acid (IBA). In the next stage, high quality calli were subcultured on MS media containing sorbitol, sucrose, maltose and mannitol (0, 5, 10 and 15 g L−1). The results indicated that 15 g L−1 maltose was able to induce the highest percentage of embryogenic callus, with an average of 88.9% on media containing 2 mg L−1 BAP and 1 mg L−1 NAA.

Investigation of callogenesis and indirect regeneration of <italic>Freesia</italic> × <italic>hybrida</italic> Bailey ‘Argenta’

Department of Horticultural Science, College of Agriculture

Folia Horticulturae

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Investigation of callogenesis and indirect regeneration of  ×  Bailey ‘Argenta’

An investigation was conducted to study the effects of explant sources, plant growth regulators, carbohydrates and light conditions on indirect cormlet...

Investigation of callogenesis and indirect regeneration of Freesia × hybrida Bailey ‘Argenta’

Published Online: Dec 30, 2015

Page range: 123 - 134

Received: Aug 19, 2014

Accepted: May 12, 2015

DOI: https://doi.org/10.1515/fhort-2015-0022

Keywords
organogenic callus, embryogenic callus, indirect cormlet formation

© 2015 Ali Pourkhaloee et al., published by De Gruyter Open

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Investigation of callogenesis and indirect regeneration of Freesia × hybrida Bailey ‘Argenta’

Published Online: Dec 30, 2015

Page range: 123 - 134

Received: Aug 19, 2014

Accepted: May 12, 2015

DOI: https://doi.org/10.1515/fhort-2015-0022

Keywordsorganogenic callus, embryogenic callus, indirect cormlet formation

© 2015 Ali Pourkhaloee et al., published by De Gruyter Open

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Keywords
organogenic callus, embryogenic callus, indirect cormlet formation