<abstract xmlns="http://www.w3.org/1999/xhtml">The study on the <italic>in vitro</italic> micropropagation of <italic>Robinia pseudoacacia</italic> L. shows the possibility of the rapid increase of the vegetative progeny using organogenesis. The culture system consists of sequential use of three media, namely, the initiation medium (MS medium supplemented with 0.6 mg · l−1 6-BA (6-benzylamino-purine), 0.1 mg · l−1 NAA (naphthalene acetic acid), proliferation medium (1/2 MS medium added with 1 mg · l−1 6-BA,) and root-induction medium (1/2 MS medium fortified with 0.2 mg · l−1 NAA). The resulting micropropagated plants, fully acclimatised to an in vivo environment, did not show any visually detectable morphological variation.In addition, we investigated the genetic stability (vegetative progeny to the donor plant) of a sample of 30 plants randomly taken from ca. 1067 micropropagated plants, by using three microsatellites loci Rops 15, Rops 16, Rops 18. Our results suggest that DNA sequence variations may occur in micropropagated plants.</abstract>

The study on the in vitro micropropagation of Robinia pseudoacacia L. shows the possibility of the rapid increase of the vegetative progeny using organogenesis. The culture system consists of sequential use of three media, namely, the initiation medium (MS medium supplemented with 0.6 mg · l−1 6-BA (6-benzylamino-purine), 0.1 mg · l−1 NAA (naphthalene acetic acid), proliferation medium (1/2 MS medium added with 1 mg · l−1 6-BA,) and root-induction medium (1/2 MS medium fortified with 0.2 mg · l−1 NAA). The resulting micropropagated plants, fully acclimatised to an in vivo environment, did not show any visually detectable morphological variation.In addition, we investigated the genetic stability (vegetative progeny to the donor plant) of a sample of 30 plants randomly taken from ca. 1067 micropropagated plants, by using three microsatellites loci Rops 15, Rops 16, Rops 18. Our results suggest that DNA sequence variations may occur in micropropagated plants.

Micropropagation of black locust (<italic>Robinia pseudoacacia</italic> L.) and genetic stability of long term cultivated plants

Forest Research Institute, Department of Silviculture and Genetics of Forest Trees, Sękocin Stary, Braci Leśnej 3, 05-090 Raszyn, Poland, phone: +48 22 7150481

Instituto Politécnico de Coimbra, Escola Superior Agrária, Bencanta, 3045-601 Coimbra, Portugal

Folia Forestalia Polonica

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Micropropagation of black locust ( L.) and genetic stability of long term cultivated plants

The study on the in vitro micropropagation of Robinia pseudoacacia L. shows the possibility of the rapid increase of the vegetative progeny using...

Micropropagation of black locust (Robinia pseudoacacia L.) and genetic stability of long term cultivated plants

Published Online: Apr 30, 2016

Page range: 13 - 19

Received: Nov 16, 2015

Accepted: Feb 01, 2016

DOI: https://doi.org/10.1515/ffp-2016-0002

Keywords
black locust, micropropagation, microsatellites, genetic stability, tissue cultures

© 2016 Iwona Szyp-Borowska et al., published by De Gruyter Open

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Micropropagation of black locust (Robinia pseudoacacia L.) and genetic stability of long term cultivated plants

Published Online: Apr 30, 2016

Page range: 13 - 19

Received: Nov 16, 2015

Accepted: Feb 01, 2016

DOI: https://doi.org/10.1515/ffp-2016-0002

Keywordsblack locust, micropropagation, microsatellites, genetic stability, tissue cultures

© 2016 Iwona Szyp-Borowska et al., published by De Gruyter Open

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Keywords
black locust, micropropagation, microsatellites, genetic stability, tissue cultures