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This paper provides a list of 40 vascular plant species recorded in 2015–2017 in the Polish part of the Lithuanian Lakeland (north-eastern Poland), using the ATPOL cartogram method. Two native taxa Erigeron acris subsp. droebachiensis and Rorippa austriaca, one established alien Atriplex tatarica and one casual alien Aronia × prunifolia are reported as new to the flora of the region.