<abstract xmlns="http://www.w3.org/1999/xhtml"><p>The purpose of this study was to examine the effects of antioxidants, including vitamin C, chitosan or a combination of both, on oxidative stress markers in muscles, as edible tissues of fish, exposed to paraquat. Fish exposed to 0.02 mg/L paraquat for 21 days were fed different diets: a normal diet, a diet containing chitosan (1000 mg/kg diet), a diet with vitamin C (1000 mg/kg diet) or both vitamin C and chitosan. Oxidative stress markers, including the activity of catalase, total antioxidant and malondialdehyde (MDA), as well as biochemical parameters including the activity of aspartate aminotransferase (AST), alanine aminotransferase (ALT), creatine phosphokinase (CPK), lactate dehydrogenase (LDH) and acetylcholinesterase (AChE), were measured in muscles. Fish exposure to paraquat increased LDH, CPK, catalase and MDA activity significantly, while it significantly decreased AST, ALT and AChE activity and total antioxidant capacity in muscles. Administration of vitamin C, combined with chitosan, to fish exposed to paraquat was effective in regulating AChE, AST, ALT, LDH, CPK and catalase activity. A significant increase in the total antioxidant status and a significant decrease in MDA levels were observed in fish fed chitosan-vitamin C complex. In conclusion, it is suggested that combined supplementation with vitamin C and chitosan may improve the detoxification system in the muscles of fish and protect common carp from paraquat toxicity.</p></abstract>

The purpose of this study was to examine the effects of antioxidants, including vitamin C, chitosan or a combination of both, on oxidative stress markers in muscles, as edible tissues of fish, exposed to paraquat. Fish exposed to 0.02 mg/L paraquat for 21 days were fed different diets: a normal diet, a diet containing chitosan (1000 mg/kg diet), a diet with vitamin C (1000 mg/kg diet) or both vitamin C and chitosan. Oxidative stress markers, including the activity of catalase, total antioxidant and malondialdehyde (MDA), as well as biochemical parameters including the activity of aspartate aminotransferase (AST), alanine aminotransferase (ALT), creatine phosphokinase (CPK), lactate dehydrogenase (LDH) and acetylcholinesterase (AChE), were measured in muscles. Fish exposure to paraquat increased LDH, CPK, catalase and MDA activity significantly, while it significantly decreased AST, ALT and AChE activity and total antioxidant capacity in muscles. Administration of vitamin C, combined with chitosan, to fish exposed to paraquat was effective in regulating AChE, AST, ALT, LDH, CPK and catalase activity. A significant increase in the total antioxidant status and a significant decrease in MDA levels were observed in fish fed chitosan-vitamin C complex. In conclusion, it is suggested that combined supplementation with vitamin C and chitosan may improve the detoxification system in the muscles of fish and protect common carp from paraquat toxicity.

The Protective Role of Vitamin C and Chitosan Against Paraquat-induced Oxidative Stress in Muscles of Common Carp (<italic>Cyprinus carpio</italic>)

Department of Aquaculture, Faculty of Natural Resources and Environment, Behbahan Khatam Al-Anbia University of Technology,

Department of Aquaculture, Faculty of Marine Science and Technology, Hormozgan University, Bandar Abbas,

Croatian Journal of Fisheries

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

The Protective Role of Vitamin C and Chitosan Against Paraquat-induced Oxidative Stress in Muscles of Common Carp ()

The purpose of this study was to examine the effects of antioxidants, including vitamin C, chitosan or a combination of both, on oxidative stress markers in...

The Protective Role of Vitamin C and Chitosan Against Paraquat-induced Oxidative Stress in Muscles of Common Carp (Cyprinus carpio)

Published Online: Dec 23, 2016

Page range: 149 - 158

Received: Mar 22, 2016

Accepted: Sep 29, 2016

DOI: https://doi.org/10.1515/cjf-2016-0023

Keywords
Antioxidants, Chitosan, Vitamin C, Paraquat, Biomarkers, Oxidative stress, Muscle

© 2016 Zeinab Sharifinasab et al., published by De Gruyter Open

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

The Protective Role of Vitamin C and Chitosan Against Paraquat-induced Oxidative Stress in Muscles of Common Carp (Cyprinus carpio)

Published Online: Dec 23, 2016

Page range: 149 - 158

Received: Mar 22, 2016

Accepted: Sep 29, 2016

DOI: https://doi.org/10.1515/cjf-2016-0023

KeywordsAntioxidants, Chitosan, Vitamin C, Paraquat, Biomarkers, Oxidative stress, Muscle

© 2016 Zeinab Sharifinasab et al., published by De Gruyter Open

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Keywords
Antioxidants, Chitosan, Vitamin C, Paraquat, Biomarkers, Oxidative stress, Muscle