<abstract xmlns="http://www.w3.org/1999/xhtml"><p>Choosing the appropriate method for differentiation of <italic>Staphylococcus aureus</italic> strains is important for effective diagnostics and epidemiological investigations. Despite the fact that the results of phenotypic methods are strongly dependent on environmental conditions, they can still be useful in the investigation of epidemic strains of S. aureus. In this article, the potential application of commonly used phenotypic methods in epidemiological studies of <italic>S. aureus</italic> was analysed. Advantages and disadvantages of methods such as biotyping, serotyping, phage typing, AST (Antimicrobial Susceptibility Testing), SDS-PAGE (Sodium Dodecyl Sulphate Polyacryl Gel Electrophoresis), MLEE (Multilocus Enzyme Electrophoresis) and MALDI-TOF MS (Matrix-Assisted Laser Desorption/Ionization Time-Of-Flight Mass Spectroscopy) were also discussed. Finally, phenotypic techniques were compared in terms of their discriminatory potential, typeability of isolates, time of analysis, reproducibility, ease of performance and ease of results interpretation.</p></abstract>

Choosing the appropriate method for differentiation of Staphylococcus aureus strains is important for effective diagnostics and epidemiological investigations. Despite the fact that the results of phenotypic methods are strongly dependent on environmental conditions, they can still be useful in the investigation of epidemic strains of S. aureus. In this article, the potential application of commonly used phenotypic methods in epidemiological studies of S. aureus was analysed. Advantages and disadvantages of methods such as biotyping, serotyping, phage typing, AST (Antimicrobial Susceptibility Testing), SDS-PAGE (Sodium Dodecyl Sulphate Polyacryl Gel Electrophoresis), MLEE (Multilocus Enzyme Electrophoresis) and MALDI-TOF MS (Matrix-Assisted Laser Desorption/Ionization Time-Of-Flight Mass Spectroscopy) were also discussed. Finally, phenotypic techniques were compared in terms of their discriminatory potential, typeability of isolates, time of analysis, reproducibility, ease of performance and ease of results interpretation.

Overview of phenotypic methods used for differentiation of <italic>Staphylococcus aureus</italic>

Department of Pharmaceutical Microbiology with Laboratory for Microbiological Diagnostics

Current Issues in Pharmacy and Medical Sciences

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 License.

Overview of phenotypic methods used for differentiation of 

Choosing the appropriate method for differentiation of Staphylococcus aureus strains is important for effective diagnostics and epidemiological investigations....

Overview of phenotypic methods used for differentiation of Staphylococcus aureus

Published Online: Oct 29, 2018

Page range: 117 - 121

Received: Feb 06, 2018

Accepted: Mar 29, 2018

DOI: https://doi.org/10.1515/cipms-2018-0023

Keywords
phenotypic methods, differentiation, typing

© 2018 Martyna Kasela et al., published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 License.

Overview of phenotypic methods used for differentiation of Staphylococcus aureus

Published Online: Oct 29, 2018

Page range: 117 - 121

Received: Feb 06, 2018

Accepted: Mar 29, 2018

DOI: https://doi.org/10.1515/cipms-2018-0023

Keywordsphenotypic methods, differentiation, typing

© 2018 Martyna Kasela et al., published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 License.

Keywords
phenotypic methods, differentiation, typing