<abstract xmlns="http://www.w3.org/1999/xhtml"><p> A rapid, simple and sensitive capillary zone electrophoresis method was developed for the simultaneous determination of amlodipine besylate and telmisartan, in fixed-dose combinations, through the utilization of a UV photodiode array detector. Electrophoretic parameters such as buffer concentration and pH, system temperature, applied voltage and injection parameters, were optimized in order to improve the efficiency of the separation. The best results were obtained when employing fused silica capillary (48 cm length X 50 μm ID) and 50 mM phosphate buffer electrolyte at pH 4.50, + 25 kV applied voltage, as well as 25°C system temperature. The separation was achieved in approximately 3 minutes, with a resolution of 4.90, while the order of migration was amlodipine followed by telmisartan. The analytical performance of the method was verified with regard to linearity, precision and robustness. In addition, the limit of detection and the quantification were calculated.</p></abstract>

A rapid, simple and sensitive capillary zone electrophoresis method was developed for the simultaneous determination of amlodipine besylate and telmisartan, in fixed-dose combinations, through the utilization of a UV photodiode array detector. Electrophoretic parameters such as buffer concentration and pH, system temperature, applied voltage and injection parameters, were optimized in order to improve the efficiency of the separation. The best results were obtained when employing fused silica capillary (48 cm length X 50 μm ID) and 50 mM phosphate buffer electrolyte at pH 4.50, + 25 kV applied voltage, as well as 25°C system temperature. The separation was achieved in approximately 3 minutes, with a resolution of 4.90, while the order of migration was amlodipine followed by telmisartan. The analytical performance of the method was verified with regard to linearity, precision and robustness. In addition, the limit of detection and the quantification were calculated.

Simultaneous determination of amlodipine and telmisartan from pharmaceutical products by way of capillary electrophoresis

Department of Pharmaceutical Chemistry, Faculty of Pharmacy, University of Medicine and Pharmacy Tîrgu Mureş, Gh. Marinescu 38, 540139 Tîrgu Mureş, Romania

Current Issues in Pharmacy and Medical Sciences

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 License.

A rapid, simple and sensitive capillary zone electrophoresis method was developed for the simultaneous determination of amlodipine besylate and telmisartan, in...

Simultaneous determination of amlodipine and telmisartan from pharmaceutical products by way of capillary electrophoresis

Published Online: May 06, 2016

Page range: 42 - 46

Received: Nov 16, 2016

Accepted: Mar 02, 2016

DOI: https://doi.org/10.1515/cipms-2016-0010

Keywords
fixed-dose combinations, Amlodipine, Telmisartan, capillary electrophoresis, simultaneous determination

© 2016

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 License.

Simultaneous determination of amlodipine and telmisartan from pharmaceutical products by way of capillary electrophoresis

Published Online: May 06, 2016

Page range: 42 - 46

Received: Nov 16, 2016

Accepted: Mar 02, 2016

DOI: https://doi.org/10.1515/cipms-2016-0010

Keywordsfixed-dose combinations, Amlodipine, Telmisartan, capillary electrophoresis, simultaneous determination

© 2016

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 License.

Keywords
fixed-dose combinations, Amlodipine, Telmisartan, capillary electrophoresis, simultaneous determination