<abstract xmlns="http://www.w3.org/1999/xhtml"> Daptomycin is the first antibiotic in a new class of cyclic lipopeptides, active in vitro against Gram - positive cocci. The mechanism of action is a rapid depolarization of the bacterial membrane potential, loss of cytoplasmic contents, mainly K+ ions and inhibition of protein, DNA and RNA synthesis, followed by the bacterial cell death. There were investigated 112 strains of Gram-positive cocci recommended for daptomycin susceptibility testing. Since the main clinical indications of daptomycin are the severe skin and soft tissue infections caused by methicillin-resistant Staphylococcus aureus, vancomicin-resistant enterococci and penicillinresistant Streptococcus spp, we tested daptomycin activity only in these species. The susceptibility tests was performed by Kirby- Bauer disc-diffusion method on Muller-Hinton agar supplemented with Ca 50 mg / ml. (CAMH - Ca 2 + ) as recommended by CLSI. During the period in which we conducted the study we isolated 112 strains of Gram-positive cocci, which we tested sensitivity to daptomycin. Resistance to Daptomycin of Staphylococcus aureus (4.11%) was lower than resistance to vancomycin (6.76%), but higher than the resistance to teicoplanin (2.71%) and linezolid (1.38%). In our study we did not identify daptomycin resistant strains of Enterococcus spp. In severe infections that require more aggressive treatment using active antibiotics on MRSA, daptomycin is a valid therapeutic option, but a susceptibility test is required to ensure effective indication. There were no daptomycin resistant Enterococcus spp. strains </abstract>

Daptomycin is the first antibiotic in a new class of cyclic lipopeptides, active in vitro against Gram - positive cocci. The mechanism of action is a rapid depolarization of the bacterial membrane potential, loss of cytoplasmic contents, mainly K+ ions and inhibition of protein, DNA and RNA synthesis, followed by the bacterial cell death. There were investigated 112 strains of Gram-positive cocci recommended for daptomycin susceptibility testing. Since the main clinical indications of daptomycin are the severe skin and soft tissue infections caused by methicillin-resistant Staphylococcus aureus, vancomicin-resistant enterococci and penicillinresistant Streptococcus spp, we tested daptomycin activity only in these species. The susceptibility tests was performed by Kirby- Bauer disc-diffusion method on Muller-Hinton agar supplemented with Ca 50 mg / ml. (CAMH - Ca 2 + ) as recommended by CLSI. During the period in which we conducted the study we isolated 112 strains of Gram-positive cocci, which we tested sensitivity to daptomycin. Resistance to Daptomycin of Staphylococcus aureus (4.11%) was lower than resistance to vancomycin (6.76%), but higher than the resistance to teicoplanin (2.71%) and linezolid (1.38%). In our study we did not identify daptomycin resistant strains of Enterococcus spp. In severe infections that require more aggressive treatment using active antibiotics on MRSA, daptomycin is a valid therapeutic option, but a susceptibility test is required to ensure effective indication. There were no daptomycin resistant Enterococcus spp. strains

In vitro resistance to daptomycin of staphylococcus aureus and enterococcus spp. strains

Emergency County Clinical Hospital “Sf. Apostol Andrei” Constanţa

Faculty of Medicine, University „Ovidius” of Constanţa

ARS Medica Tomitana

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Daptomycin is the first antibiotic in a new class of cyclic lipopeptides, active in vitro against Gram - positive cocci. The mechanism of action is a rapid...

In vitro resistance to daptomycin of staphylococcus aureus and enterococcus spp. strains

Published Online: Mar 11, 2015

Page range: 195 - 199

DOI: https://doi.org/10.1515/arsm-2015-0005

Keywords
Daptomycin, resistance, Staphylococcus, Enterococcus

© by Irina Stan

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

In vitro resistance to daptomycin of staphylococcus aureus and enterococcus spp. strains

Published Online: Mar 11, 2015

Page range: 195 - 199

DOI: https://doi.org/10.1515/arsm-2015-0005

KeywordsDaptomycin, resistance, Staphylococcus, Enterococcus

© by Irina Stan

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Keywords
Daptomycin, resistance, Staphylococcus, Enterococcus