1. bookVolume 18 (2018): Issue 2 (May 2018)
Journal Details
License
Format
Journal
eISSN
2300-8733
First Published
25 Nov 2011
Publication timeframe
4 times per year
Languages
English
access type Open Access

Identification and Changes in the Seasonal Concentrations of Proteins Regulating the Biological Functions of Spermatozoa and Participating in Their Cytoskeleton Organization in Roe Deer (Capreolus Capreolus) Epididymides

Published Online: 11 May 2018
Volume & Issue: Volume 18 (2018) - Issue 2 (May 2018)
Page range: 417 - 428
Received: 04 Jul 2017
Accepted: 21 Dec 2017
Journal Details
License
Format
Journal
eISSN
2300-8733
First Published
25 Nov 2011
Publication timeframe
4 times per year
Languages
English
Abstract

In the case of animals characterized by a strongly marked seasonality of reproduction, extremely significant seem to be periodical fluctuations in the concentration of proteinaceous substances identified in tissues and fluids of epididymides, which take part in the maturation process of sperm cells. The aim of the present study was to compare the seasonal expression of identified proteins present in various regions of the mature roe deer (Capreolus capreolus) epididymides, which are regulating the biological functions of spermatozoa and participating in their cytoskeleton organization during the sperm maturation process. Epididymal tissue homogenates and epididymal fluids were analyzed by two-dimensional electrophoresis (2D-PAGE) and tandem mass spectrometry (MS/MS) to reveal 31 polypeptides with different biochemical functions. Moreover, among the identified polypeptides, twelve of them were similar to: alpha-enolase isoform 3; endoplasmic reticulum resident protein 29; calreticulin, calponin-1 isoform X1; transgelin; vimentin; tubulin; desmin; tropomyosin; actin; alpha-1 antitrypsin isoform X1 and 14-3-3 protein epsilon. Concentrations of the analyzed polypeptides, expressed in optical density units (ODU), differed significantly (P≤0.05) across the examined periods of the reproductive season. The highest ODU values of almost all analyzed proteins were observed during the rutting period. This variation in the identified proteins in the epididymal tissues and fluids of roe deer throughout the reproductive season could be indicative of their important roles in sperm maturation within the epididymis.

Keywords

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