<abstract xmlns="http://www.w3.org/1999/xhtml"><p> One of the factors determining the homeostasis of biological systems is the balance between the formation of reactive oxygen species (ROS) and the activity of the antioxidative defence system. Regarding seasonal variations in secretory activity in different parts of roe buck’s (Capreolus capreolus) epididymis, the aim of present study was to compare the expression of selected antioxidative proteins in three periods of the rutting season. Using proteomics methods (2D PAGE , tandem mass spectrometry MS/MS) 5 antioxidative enzymes were identified for the first time in different segments of the epididymis of the roe buck. The highest expression of these enzymes was found in the rutting season. Findings indicated that the antioxidative function of the buck’s epididymis, regardless of the period of the rutting season, but particularly in the rutting season, is maintained mainly because of the presence of three conservative polypeptides: glutathione S-transferase (GST), protein disulfide isomerase A3 (PDIA3), and PDIA3 precursor. Moreover, a protective role against the harmful products of redox reactions is played during the discussed periods by peroxiredoxin-2 (PRDX2), identified in the cauda of the epididymis. In the tissue of the corpus and caput of the epididymis its expression was only found in the rutting season. The expression of biliverdin reductase A (BRVA ) in the epididymis was only observed in the rutting season.</p></abstract>

One of the factors determining the homeostasis of biological systems is the balance between the formation of reactive oxygen species (ROS) and the activity of the antioxidative defence system. Regarding seasonal variations in secretory activity in different parts of roe buck’s (Capreolus capreolus) epididymis, the aim of present study was to compare the expression of selected antioxidative proteins in three periods of the rutting season. Using proteomics methods (2D PAGE , tandem mass spectrometry MS/MS) 5 antioxidative enzymes were identified for the first time in different segments of the epididymis of the roe buck. The highest expression of these enzymes was found in the rutting season. Findings indicated that the antioxidative function of the buck’s epididymis, regardless of the period of the rutting season, but particularly in the rutting season, is maintained mainly because of the presence of three conservative polypeptides: glutathione S-transferase (GST), protein disulfide isomerase A3 (PDIA3), and PDIA3 precursor. Moreover, a protective role against the harmful products of redox reactions is played during the discussed periods by peroxiredoxin-2 (PRDX2), identified in the cauda of the epididymis. In the tissue of the corpus and caput of the epididymis its expression was only found in the rutting season. The expression of biliverdin reductase A (BRVA ) in the epididymis was only observed in the rutting season.

Changes in the expression of selected antioxidative proteins in roe deer (Capreolus capreolus) epididymis in different periods of the rutting season

Department of Animal Biochemistry and Biotechnology, University of Warmia and Mazury in Olsztyn, Oczapowskiego 5, 10-718 Olsztyn,

Annals of Animal Science

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 License.

One of the factors determining the homeostasis of biological systems is the balance between the formation of reactive oxygen species (ROS) and the activity of...

Changes in the expression of selected antioxidative proteins in roe deer (Capreolus capreolus) epididymis in different periods of the rutting season

Article Category: The biology, physiology, reproduction, and health

Published Online: Apr 28, 2017

Page range: 423 - 431

Received: May 31, 2016

Accepted: Aug 11, 2016

DOI: https://doi.org/10.1515/aoas-2016-0052

Keywords
roe deer (Capreolus capreolus), epididymis, antioxidant system, proteomics

© by Władysław Kordan

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 License.

Changes in the expression of selected antioxidative proteins in roe deer (Capreolus capreolus) epididymis in different periods of the rutting season

Article Category: The biology, physiology, reproduction, and health

Published Online: Apr 28, 2017

Page range: 423 - 431

Received: May 31, 2016

Accepted: Aug 11, 2016

DOI: https://doi.org/10.1515/aoas-2016-0052

Keywordsroe deer (Capreolus capreolus), epididymis, antioxidant system, proteomics

© by Władysław Kordan

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 License.

Keywords
roe deer (Capreolus capreolus), epididymis, antioxidant system, proteomics