<abstract xmlns="http://www.w3.org/1999/xhtml"><p>Continually improved cytogenetic techniques (differential staining and high resolution banding techniques), complemented with the molecular genetics methods (FISH and PRINS), enable chromosomal mutations to be accurately identified in the karyotype of the pig (<italic>Sus scrofa</italic>). The major breeding problem are balanced mutations because of their hidden nature, as they affect the animals with normal body conformation (and normal semen parameters in boars), which transfer these aberrations to the next generations and disseminate in the population. This refers to the structural rearrangements (translocations and inversions), causing developmental abnormalities and considerably reducing fertility and productivity parameters in breeding herds, which results in substantial financial losses. Routine karyotype screening using modern cytomolecular diagnostic methods is necessary due to the potential emergence of new mutations and the rapid spread of these genetic defects in the population, especially under artificial insemination conditions.</p></abstract>

Continually improved cytogenetic techniques (differential staining and high resolution banding techniques), complemented with the molecular genetics methods (FISH and PRINS), enable chromosomal mutations to be accurately identified in the karyotype of the pig (Sus scrofa). The major breeding problem are balanced mutations because of their hidden nature, as they affect the animals with normal body conformation (and normal semen parameters in boars), which transfer these aberrations to the next generations and disseminate in the population. This refers to the structural rearrangements (translocations and inversions), causing developmental abnormalities and considerably reducing fertility and productivity parameters in breeding herds, which results in substantial financial losses. Routine karyotype screening using modern cytomolecular diagnostic methods is necessary due to the potential emergence of new mutations and the rapid spread of these genetic defects in the population, especially under artificial insemination conditions.

Molecular Cytogenetics in the Diagnostics of Balanced Chromosome Mutations in the Pig (<italic>Sus scrofa</italic>) – A Review

Department of Animal Genomics and Molecular Biology, National Research Institute of Animal Production, 32-083 Balice n. Kraków, Poland

Annals of Animal Science

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Molecular Cytogenetics in the Diagnostics of Balanced Chromosome Mutations in the Pig () – A Review

Continually improved cytogenetic techniques (differential staining and high resolution banding techniques), complemented with the molecular genetics methods...

Molecular Cytogenetics in the Diagnostics of Balanced Chromosome Mutations in the Pig (Sus scrofa) – A Review

Published Online: Aug 02, 2016

Page range: 679 - 699

Received: Aug 21, 2015

Accepted: Feb 16, 2016

DOI: https://doi.org/10.1515/aoas-2016-0008

Keywords
banding and FISH techniques, balanced chromosome mutations, meiosis, fertility of pigs

© 2016 Barbara Danielak-Czech et al., published by De Gruyter Open

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Molecular Cytogenetics in the Diagnostics of Balanced Chromosome Mutations in the Pig (Sus scrofa) – A Review

Published Online: Aug 02, 2016

Page range: 679 - 699

Received: Aug 21, 2015

Accepted: Feb 16, 2016

DOI: https://doi.org/10.1515/aoas-2016-0008

Keywordsbanding and FISH techniques, balanced chromosome mutations, meiosis, fertility of pigs

© 2016 Barbara Danielak-Czech et al., published by De Gruyter Open

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Keywords
banding and FISH techniques, balanced chromosome mutations, meiosis, fertility of pigs