<abstract xmlns="http://www.w3.org/1999/xhtml"><p>One of epigenetic features of mammalian genomes is methylation of DNA. This nucleotide modification might exert suppressive effect on gene transcription. We have described putative relevance of methylation of one of immune cells related gene (<italic>ITGAL</italic>) observed in the set of 11 equine tissues. Comparison between qualitative RT-PCR results and DNA bisulfite sequencing of investigated set of tissues pointed to potential correlations between tissue specific methylation and tissue specific transcription in <italic>ITGAL</italic> locus. These findings might be important for studies on genetic and epigenetic background of autoimmune disorders in the horse.</p></abstract>

One of epigenetic features of mammalian genomes is methylation of DNA. This nucleotide modification might exert suppressive effect on gene transcription. We have described putative relevance of methylation of one of immune cells related gene (ITGAL) observed in the set of 11 equine tissues. Comparison between qualitative RT-PCR results and DNA bisulfite sequencing of investigated set of tissues pointed to potential correlations between tissue specific methylation and tissue specific transcription in ITGAL locus. These findings might be important for studies on genetic and epigenetic background of autoimmune disorders in the horse.

The Relevance of Methylation Profiles of Equine <italic>ITGAL</italic> Gene

Department of Genomics and Molecular Biology, National Research Institute of Animal Production, 32-083 Balice n. Kraków, Poland

Annals of Animal Science

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

The Relevance of Methylation Profiles of Equine  Gene

One of epigenetic features of mammalian genomes is methylation of DNA. This nucleotide modification might exert suppressive effect on gene transcription. We...

The Relevance of Methylation Profiles of Equine ITGAL Gene

Published Online: Aug 02, 2016

Page range: 711 - 720

Received: May 29, 2015

Accepted: Nov 13, 2015

DOI: https://doi.org/10.1515/aoas-2015-0080

Keywords
DNA methylation, transcription, horses

© 2016 Tomasz Ząbek et al., published by De Gruyter Open

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

The Relevance of Methylation Profiles of Equine ITGAL Gene

Published Online: Aug 02, 2016

Page range: 711 - 720

Received: May 29, 2015

Accepted: Nov 13, 2015

DOI: https://doi.org/10.1515/aoas-2015-0080

KeywordsDNA methylation, transcription, horses

© 2016 Tomasz Ząbek et al., published by De Gruyter Open

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Keywords
DNA methylation, transcription, horses