<abstract xmlns="http://www.w3.org/1999/xhtml"><p> Nanoparticles can be an alternative for currently used viral and non-viral systems of transporting exogenous DNA into cells, and furthermore, can be an effective way to produce transgenic animals. The possibility of linking them with proteins, lipids and of adding ligands enables improved transfection by making the crossing of membranes and the breaking of the endosomal barrier more efficient. Additionally, by the addition of magnetic particles it is possible to amend the intracellular kinetics of nanoparticle-DNA complexes. This review considers the use of nanoparticles to transfect cells and embryos and their possible application as a non-viral vector in animal transgenesis.</p></abstract>

Nanoparticles can be an alternative for currently used viral and non-viral systems of transporting exogenous DNA into cells, and furthermore, can be an effective way to produce transgenic animals. The possibility of linking them with proteins, lipids and of adding ligands enables improved transfection by making the crossing of membranes and the breaking of the endosomal barrier more efficient. Additionally, by the addition of magnetic particles it is possible to amend the intracellular kinetics of nanoparticle-DNA complexes. This review considers the use of nanoparticles to transfect cells and embryos and their possible application as a non-viral vector in animal transgenesis.

Nanoparticles as a Tool for Transfection and Transgenesis – a Review

Department of Biotechnology of Animal Reproduction, National Research Institute of Animal Production, 32-083 Balice n. Kraków, Poland

Annals of Animal Science

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 License.

{"article-title":"Nanoparticles as a Tool for Transfection and Transgenesis \u2013 a Review"}

Nanoparticles can be an alternative for currently used viral and non-viral systems of transporting exogenous DNA into cells, and furthermore, can be an...

Nanoparticles as a Tool for Transfection and Transgenesis – a Review

Article Category: Review

Published Online: Jan 23, 2016

Page range: 53 - 64

Received: Mar 09, 2015

Accepted: Oct 28, 2015

DOI: https://doi.org/10.1515/aoas-2015-0077

Keywords
transgenesis, nanoparticles, transfection

© by Natalia Dzięgiel

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 License.

Nanoparticles as a Tool for Transfection and Transgenesis – a Review

Article Category: Review

Published Online: Jan 23, 2016

Page range: 53 - 64

Received: Mar 09, 2015

Accepted: Oct 28, 2015

DOI: https://doi.org/10.1515/aoas-2015-0077

Keywordstransgenesis, nanoparticles, transfection

© by Natalia Dzięgiel

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 License.

Keywords
transgenesis, nanoparticles, transfection