<abstract xmlns="http://www.w3.org/1999/xhtml"><p> Haptoglobin (Hp) is a protein secreted mainly from the liver to bind hemoglobin and thus prevents iron loss and oxidative stress. Bovine Hp in blood increases during diseases such as mastitis and fatty liver syndrome. This study was conducted to examine the expression and regulation of Hp in differentiated mature bovine adipocytes. Hp expression was confirmed in various tissues and in mature adipocytes but not in the stromal vascular cell fraction using qualitative RT -PCR and quantitative northern blot analysis, respectively. Intravenous injection of Holstein cows with recombinant bovine tumor necrosis factor-α (rbTNF-α) and interferon gamma (rbIFN-γ) significantly increased the serum concentrations of Hp. When mature differentiated bovine adipocytes were incubated with short chain fatty acids, cytokines and hormones, Hp expression was altered. Acetate, butyrate and propionate at a dose of 1 mM significantly up-regulated Hp mRNA abundance. Lipopolysaccharide (LPS) and TNF-α also significantly up-regulated Hp mRNA abundance, while troglitazone and norepinephrine down-regulated it. Our study confirmed expression of Hp in differentiated mature adipocytes and identified LPS, TNF-α, short chain fatty acids, as regulators of Hp mRNA expression in these cells.</p></abstract>

Haptoglobin (Hp) is a protein secreted mainly from the liver to bind hemoglobin and thus prevents iron loss and oxidative stress. Bovine Hp in blood increases during diseases such as mastitis and fatty liver syndrome. This study was conducted to examine the expression and regulation of Hp in differentiated mature bovine adipocytes. Hp expression was confirmed in various tissues and in mature adipocytes but not in the stromal vascular cell fraction using qualitative RT -PCR and quantitative northern blot analysis, respectively. Intravenous injection of Holstein cows with recombinant bovine tumor necrosis factor-α (rbTNF-α) and interferon gamma (rbIFN-γ) significantly increased the serum concentrations of Hp. When mature differentiated bovine adipocytes were incubated with short chain fatty acids, cytokines and hormones, Hp expression was altered. Acetate, butyrate and propionate at a dose of 1 mM significantly up-regulated Hp mRNA abundance. Lipopolysaccharide (LPS) and TNF-α also significantly up-regulated Hp mRNA abundance, while troglitazone and norepinephrine down-regulated it. Our study confirmed expression of Hp in differentiated mature adipocytes and identified LPS, TNF-α, short chain fatty acids, as regulators of Hp mRNA expression in these cells.

Expression and Regulation of Haptoglobin in Tissues and in Differentiated Bovine Adipocytes

Medical Laboratory Department, College of Applied Medical Sciences, Taif University, Turaba, Saudi Arabia

Department of Biochemistry, College of Veterinary Medicine, Benha University, Moshtohor, P.O. 13736, Egypt

Department of Physiology, Faculty of Veterinary Medicine, Zagazig University, Egypt

Department of Medical Biotechnology, Faculty of Applied Medical Sciences, Turabah, Taif University, Saudi Arabia

Department of Genetics, Faculty of Agriculture, Ain Shams University, Cairo, Egypt

Annals of Animal Science

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Haptoglobin (Hp) is a protein secreted mainly from the liver to bind hemoglobin and thus prevents iron loss and oxidative stress. Bovine Hp in blood increases...

Expression and Regulation of Haptoglobin in Tissues and in Differentiated Bovine Adipocytes

Published Online: Apr 23, 2015

Page range: 419 - 431

Received: May 13, 2014

Accepted: Dec 01, 2014

DOI: https://doi.org/10.1515/aoas-2015-0004

Keywords
adipocytes, bovine haptoglobin, expression, regulation

© by Mohamed Mohamed Soliman

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Expression and Regulation of Haptoglobin in Tissues and in Differentiated Bovine Adipocytes

Published Online: Apr 23, 2015

Page range: 419 - 431

Received: May 13, 2014

Accepted: Dec 01, 2014

DOI: https://doi.org/10.1515/aoas-2015-0004

Keywordsadipocytes, bovine haptoglobin, expression, regulation

© by Mohamed Mohamed Soliman

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Keywords
adipocytes, bovine haptoglobin, expression, regulation