<abstract xmlns="http://www.w3.org/1999/xhtml"><p>The association of phytoplasma was investigated in symptomatic tomato (<italic>Solanum lycopersicum</italic> L.), eggplant (<italic>Solanum melongen</italic> L.), mallow (<italic>Malva</italic> spp.) and Bermuda grass (<italic>Cynodon dactylon</italic> L.) plants exhibiting witches’ broom and white leaf diseases, respectively. Total DNA was extracted from tomato (n=3), eggplant (n=2), mallow (n=2) and Bermuda grass (n=8) samples. Direct polymerase chain reaction (PCR) was performed using P1/P7 primer set, then PCR products were sequenced. Sequences obtained from tomato, eggplant and mallow shared 99% maximum nucleotide identity with phytoplasma belonging to subgroup 16SrII-D, and resulted therefore ‘<italic>Candidatus</italic> Phytoplasma australasia’-related. Sequences obtained from Bermuda grass showed 100% maximum nucleotide identity to 16SrXIV-A subgroup and were ‘<italic>Ca</italic>. P. cynodontis’-related. The study presents the first molecular confirmation and sequence data of presence of ‘<italic>Ca.</italic> P. australasia’ and ‘<italic>Ca</italic>. P. cynodontis’ in Iraq.</p></abstract>

The association of phytoplasma was investigated in symptomatic tomato (Solanum lycopersicum L.), eggplant (Solanum melongen L.), mallow (Malva spp.) and Bermuda grass (Cynodon dactylon L.) plants exhibiting witches’ broom and white leaf diseases, respectively. Total DNA was extracted from tomato (n=3), eggplant (n=2), mallow (n=2) and Bermuda grass (n=8) samples. Direct polymerase chain reaction (PCR) was performed using P1/P7 primer set, then PCR products were sequenced. Sequences obtained from tomato, eggplant and mallow shared 99% maximum nucleotide identity with phytoplasma belonging to subgroup 16SrII-D, and resulted therefore ‘Candidatus Phytoplasma australasia’-related. Sequences obtained from Bermuda grass showed 100% maximum nucleotide identity to 16SrXIV-A subgroup and were ‘Ca. P. cynodontis’-related. The study presents the first molecular confirmation and sequence data of presence of ‘Ca. P. australasia’ and ‘Ca. P. cynodontis’ in Iraq.

Molecular detection of ‘<italic>Candidatus</italic> Phytoplasma australasia’ and ‘<italic>Ca.</italic> P. cynodontis’ in Iraq

Agriculture (Pol'nohospodárstvo)

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Molecular detection of ‘ Phytoplasma australasia’ and ‘ P. cynodontis’ in Iraq

The association of phytoplasma was investigated in symptomatic tomato (Solanum lycopersicum L.), eggplant (Solanum melongen L.), mallow (Malva spp.) and...

Molecular detection of ‘Candidatus Phytoplasma australasia’ and ‘Ca. P. cynodontis’ in Iraq

Published Online: Oct 25, 2017

Page range: 112 - 119

Received: May 27, 2017

DOI: https://doi.org/10.1515/agri-2017-0011

Keywords
witches’ broom, white leaf, tomato, eggplant, Bermuda grass

© 2017 Nawres Abdulelah Sadeq Alkuwaiti et al., published by De Gruyter Open

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Molecular detection of ‘Candidatus Phytoplasma australasia’ and ‘Ca. P. cynodontis’ in Iraq

Published Online: Oct 25, 2017

Page range: 112 - 119

Received: May 27, 2017

DOI: https://doi.org/10.1515/agri-2017-0011

Keywordswitches’ broom, white leaf, tomato, eggplant, Bermuda grass

© 2017 Nawres Abdulelah Sadeq Alkuwaiti et al., published by De Gruyter Open

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Keywords
witches’ broom, white leaf, tomato, eggplant, Bermuda grass