<abstract xmlns="http://www.w3.org/1999/xhtml"><p>The aim of the work was to find an experimental model suitable for the study of endothelial dysfunction induced by MS. We used hypertriglyceridemic rats (HTG) that were fed a hypercholesterolemic diet of different composition and duration: a 6-week administration of standard diet with an addition of cholesterol and fat (HTGChol) and a three-month administration of the same diet with an addition of fructose (HTGCholF). We investigated the effect of different diets on aortic endothelial function. The standard diet fed Wistar (W) and HTG rats served as controls. Decision for addition of fructose to HTGChol was done based on in vitro experiments evaluating the effect of high concentration of saccharide in the incubation solution on aortic endothelial function. This intervention caused significant deterioration of relaxation induced by acetylcholine (ACh). While in HTGChol, we did not find significant differences in the function of the aorta compared to W or HTG rats, adding of fructose to high fat diet and prolonging its administration resulted in significantly impaired endothelium-dependent relaxation. It seems that such a model is suitable for the study of endothelial dysfunction in MS and the effect of substances that may protect the endothelium.</p></abstract>

The aim of the work was to find an experimental model suitable for the study of endothelial dysfunction induced by MS. We used hypertriglyceridemic rats (HTG) that were fed a hypercholesterolemic diet of different composition and duration: a 6-week administration of standard diet with an addition of cholesterol and fat (HTGChol) and a three-month administration of the same diet with an addition of fructose (HTGCholF). We investigated the effect of different diets on aortic endothelial function. The standard diet fed Wistar (W) and HTG rats served as controls. Decision for addition of fructose to HTGChol was done based on in vitro experiments evaluating the effect of high concentration of saccharide in the incubation solution on aortic endothelial function. This intervention caused significant deterioration of relaxation induced by acetylcholine (ACh). While in HTGChol, we did not find significant differences in the function of the aorta compared to W or HTG rats, adding of fructose to high fat diet and prolonging its administration resulted in significantly impaired endothelium-dependent relaxation. It seems that such a model is suitable for the study of endothelial dysfunction in MS and the effect of substances that may protect the endothelium.

Endothelial dysfunction in experimental models of metabolic syndrome − effect of fructose

Slovak Academy of Sciences, Institute of Experimental Pharmacology and Toxicology

Slovak Academy of Sciences, Institute of Heart Research

Jessenius Faculty of Medicine in Martin, Department of Pharmacology

European Pharmaceutical Journal

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

The aim of the work was to find an experimental model suitable for the study of endothelial dysfunction induced by MS. We used hypertriglyceridemic rats (HTG)...

Endothelial dysfunction in experimental models of metabolic syndrome − effect of fructose

Published Online: Nov 10, 2017

Page range: 4 - 6

Received: Jun 22, 2016

Accepted: Jul 19, 2016

DOI: https://doi.org/10.1515/afpuc-2017-0014

Keywords
Endothelium, Metabolic Syndrome, HTG

© 2017 Kaprinay B. et al., published by De Gruyter Open

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Endothelial dysfunction in experimental models of metabolic syndrome − effect of fructose

Published Online: Nov 10, 2017

Page range: 4 - 6

Received: Jun 22, 2016

Accepted: Jul 19, 2016

DOI: https://doi.org/10.1515/afpuc-2017-0014

KeywordsEndothelium, Metabolic Syndrome, HTG

© 2017 Kaprinay B. et al., published by De Gruyter Open

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Keywords
Endothelium, Metabolic Syndrome, HTG