<abstract xmlns="http://www.w3.org/1999/xhtml"><p> The presence of bovine parainfluenza virus type 3 (BPIV3) was examined in 119 nasal swabs collected from cattle with severe respiratory infection. All samples were conducted for virus isolation on the MDBK cell line. The cytopathic effect was observed after 48h to 72h in cells inoculated with eight samples (8/119; 6.7%). The confirmation of isolated strains of BPIV3 was done by the virus-neutralization test. In addition, all samples of bovine nasal swabs were also examined for the presence of BPIV3 virus using RT-PCR with primers specific for the part of HN gene. The presence of BPIV3 was detected in eight samples (8/119; 6.7%) that were also positive upon virus isolation. The molecular characterization based on nucleotide sequencing of the part of the HN gene showed that all BPIV3 isolates belonged to genotype C of BPIV3. They branched in one distinct cluster with three different branches, but these branches were very similar to each other (98.1% to 99.8%). Serbian BPIV3c isolates were most similar to the Chinese BPIV3c isolates SD0805, SD0809 and SD0835 (from 97.92% to 99.7%), and to South Korean (12Q061), Japanese (HS9) and American (TVMDL16 and TVMDL20) BPIV3c strains (from 97.1% to 98.8%), and distinct from American (TVMDL15and TVMDL17) and Australian (Q5592) BPI3V genotype B strains (only 79.9% to 82.3% similarity), as well as from the genotype A BPIV3 strains from different countries published in GenBank.</p></abstract>

The presence of bovine parainfluenza virus type 3 (BPIV3) was examined in 119 nasal swabs collected from cattle with severe respiratory infection. All samples were conducted for virus isolation on the MDBK cell line. The cytopathic effect was observed after 48h to 72h in cells inoculated with eight samples (8/119; 6.7%). The confirmation of isolated strains of BPIV3 was done by the virus-neutralization test. In addition, all samples of bovine nasal swabs were also examined for the presence of BPIV3 virus using RT-PCR with primers specific for the part of HN gene. The presence of BPIV3 was detected in eight samples (8/119; 6.7%) that were also positive upon virus isolation. The molecular characterization based on nucleotide sequencing of the part of the HN gene showed that all BPIV3 isolates belonged to genotype C of BPIV3. They branched in one distinct cluster with three different branches, but these branches were very similar to each other (98.1% to 99.8%). Serbian BPIV3c isolates were most similar to the Chinese BPIV3c isolates SD0805, SD0809 and SD0835 (from 97.92% to 99.7%), and to South Korean (12Q061), Japanese (HS9) and American (TVMDL16 and TVMDL20) BPIV3c strains (from 97.1% to 98.8%), and distinct from American (TVMDL15and TVMDL17) and Australian (Q5592) BPI3V genotype B strains (only 79.9% to 82.3% similarity), as well as from the genotype A BPIV3 strains from different countries published in GenBank.

Isolation and Molecular Detection of Bovine Parainfluenza Virus Type 3 in Cattle in Serbia

Scientific Institute of Veterinary Medicine of Serbia, Vojvode Toze 14, Belgrade,

Virology Department, Institute of Microbiology and Immunology, School of Medicine, University of Belgrade, Dr Subotica 8, Belgrade,

Department of Microbiology, Faculty of veterinary medicine University of Belgrade, Bul. Oslobođenja 18, Belgrade,

Specialized Veterinary Laboratory, Bul. Džordža Vašingtona, bb, Podgorica,

Medicines and Medical Devices Agency of Serbia, Vojvode Stepe 458, Belgrade,

Virology department, Scientific Veterinary Institute „Novi Sad“, Rumenački put 20, Novi Sad,

Acta Veterinaria

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 License.

The presence of bovine parainfluenza virus type 3 (BPIV3) was examined in 119 nasal swabs collected from cattle with severe respiratory infection. All samples...

Isolation and Molecular Detection of Bovine Parainfluenza Virus Type 3 in Cattle in Serbia

Published Online: Dec 30, 2016

Page range: 509 - 519

Received: Feb 14, 2016

Accepted: Nov 15, 2016

DOI: https://doi.org/10.1515/acve-2016-0044

Keywords
bovine parainfluenza virus type 3, virus isolation, RT-PCR, Serbia

© by Tamaš Petrović

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 License.

Isolation and Molecular Detection of Bovine Parainfluenza Virus Type 3 in Cattle in Serbia

Published Online: Dec 30, 2016

Page range: 509 - 519

Received: Feb 14, 2016

Accepted: Nov 15, 2016

DOI: https://doi.org/10.1515/acve-2016-0044

Keywordsbovine parainfluenza virus type 3, virus isolation, RT-PCR, Serbia

© by Tamaš Petrović

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 License.

Keywords
bovine parainfluenza virus type 3, virus isolation, RT-PCR, Serbia