<abstract xmlns="http://www.w3.org/1999/xhtml"><p> This study investigated whether the L-arginine-NO system participates in the analgesic effect of flunixin meglumine in the rat. Hyperalgesia was induced by intraplantar (i.pl.) administration of carrageenan (500 μg) into the rat’s hind paw. Electronic von Frey apparatus was used to determine paw withdrawal threshold induced by pressure as the painful stimulus, measured in grams (g). Flunixin meglumine (FM; 0.09-0.1 mg/kg; s.c.) and N<sup>G</sup>-nitro-L-arginine methyl ester (L-NAME; 10 mg/kg; i.p.), given separately as a pre-treatment, i.e. 15 min before i.pl. injection of carrageenan, produced a significant antinociception. When FM (0.09 mg/kg) and a sub-effective dose of L-NAME (5 mg/kg) were co-administered, the antinociceptive effect was significantly increased in comparison with the effect of FM alone. L-arginine (L-ARG;10 mg/kg; i.p.) itself did not produce significant effect on carrageenan-induced hyperalgesia, but significantly reduced the antinociceptive effects of both FM and FM + L-NAME combination. The inhibition of the production of NO might be involved in the mechanism of the analgesic effect of FM.</p></abstract>

This study investigated whether the L-arginine-NO system participates in the analgesic effect of flunixin meglumine in the rat. Hyperalgesia was induced by intraplantar (i.pl.) administration of carrageenan (500 μg) into the rat’s hind paw. Electronic von Frey apparatus was used to determine paw withdrawal threshold induced by pressure as the painful stimulus, measured in grams (g). Flunixin meglumine (FM; 0.09-0.1 mg/kg; s.c.) and NG-nitro-L-arginine methyl ester (L-NAME; 10 mg/kg; i.p.), given separately as a pre-treatment, i.e. 15 min before i.pl. injection of carrageenan, produced a significant antinociception. When FM (0.09 mg/kg) and a sub-effective dose of L-NAME (5 mg/kg) were co-administered, the antinociceptive effect was significantly increased in comparison with the effect of FM alone. L-arginine (L-ARG;10 mg/kg; i.p.) itself did not produce significant effect on carrageenan-induced hyperalgesia, but significantly reduced the antinociceptive effects of both FM and FM + L-NAME combination. The inhibition of the production of NO might be involved in the mechanism of the analgesic effect of FM.

L-Arginine-No System Participates in the Analgesic Effect of Flunixin Meglumine in the Rat

Faculty of Veterinary Medicine University of Belgrade, Bul. Oslobodjenja 18, 11000 Belgrade, Serbia

Medical Faculty University of Belgrade, Dr. Subotica 8, 11000 Belgrade, Serbia

Acta Veterinaria

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 License.

This study investigated whether the L-arginine-NO system participates in the analgesic effect of flunixin meglumine in the rat. Hyperalgesia was induced by...

L-Arginine-No System Participates in the Analgesic Effect of Flunixin Meglumine in the Rat

Published Online: Mar 23, 2016

Page range: 103 - 114

Received: Sep 15, 2015

Accepted: Dec 11, 2015

DOI: https://doi.org/10.1515/acve-2016-0008

Keywords
Flunixin meglumine, L-NAME, L-arginine, carrageenan, hyperalgesia

© by Mirjana Milovanović

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 License.

L-Arginine-No System Participates in the Analgesic Effect of Flunixin Meglumine in the Rat

Published Online: Mar 23, 2016

Page range: 103 - 114

Received: Sep 15, 2015

Accepted: Dec 11, 2015

DOI: https://doi.org/10.1515/acve-2016-0008

KeywordsFlunixin meglumine, L-NAME, L-arginine, carrageenan, hyperalgesia

© by Mirjana Milovanović

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 License.

Keywords
Flunixin meglumine, L-NAME, L-arginine, carrageenan, hyperalgesia