<abstract xmlns="http://www.w3.org/1999/xhtml"><p>In the current paper, an HPLC/UV method was developed and validated for determination of wogonin in plasma. Considerable attention was paid to the preparation of standard samples and factors affecting drug distribution. A preparation procedure was devised to simulate the conditions the drug is expected to experience <italic>in vivo</italic> while pointing to the shortcomings of previously published methods. The method was validated according to the FDA regulations and showed to be highly efficient and capable of extracting the drug and IS from the plasma accurately and precisely within the specified range of 50–500 ng mL<sup>−1</sup>. Further, the standard sample preparation of this method can be used as a guideline for other methods, particularly when highly hydrophobic drugs with considerable protein binding are involved and could be valuable in the field of bioanalysis to improve the reliability of methods.</p></abstract>

In the current paper, an HPLC/UV method was developed and validated for determination of wogonin in plasma. Considerable attention was paid to the preparation of standard samples and factors affecting drug distribution. A preparation procedure was devised to simulate the conditions the drug is expected to experience in vivo while pointing to the shortcomings of previously published methods. The method was validated according to the FDA regulations and showed to be highly efficient and capable of extracting the drug and IS from the plasma accurately and precisely within the specified range of 50–500 ng mL−1. Further, the standard sample preparation of this method can be used as a guideline for other methods, particularly when highly hydrophobic drugs with considerable protein binding are involved and could be valuable in the field of bioanalysis to improve the reliability of methods.

Development of a new HPLC method for wogonin in rat plasma: Compatibility of standard and test samples

Department of Hematology and Oncology (Key Department of Jiangsu Medicine)

State Key Laboratory of Natural Medicines

Jiangsu Key Laboratory of Carcinogenesis and Intervention China Pharmaceutical University

Acta Pharmaceutica

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

In the current paper, an HPLC/UV method was developed and validated for determination of wogonin in plasma. Considerable attention was paid to the preparation...

Development of a new HPLC method for wogonin in rat plasma: Compatibility of standard and test samples

Published Online: Aug 30, 2017

Page range: 373 - 384

Accepted: May 24, 2017

DOI: https://doi.org/10.1515/acph-2017-0029

Keywords
wogonin, HPLC/UV, standard sample preparation, sample compatibility

© 2017 Jumah Masoud Mohammad Salmani et al., published by De Gruyter Open

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Development of a new HPLC method for wogonin in rat plasma: Compatibility of standard and test samples

Published Online: Aug 30, 2017

Page range: 373 - 384

Accepted: May 24, 2017

DOI: https://doi.org/10.1515/acph-2017-0029

Keywordswogonin, HPLC/UV, standard sample preparation, sample compatibility

© 2017 Jumah Masoud Mohammad Salmani et al., published by De Gruyter Open

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Keywords
wogonin, HPLC/UV, standard sample preparation, sample compatibility