<abstract xmlns="http://www.w3.org/1999/xhtml"> Olive leaf extract is characterized by a high content of polyphenols (oleuropein, hydroxytyrosol and their derivatives), which is associated with its therapeutic properties. The objective of the present research was to evaluate the antifungal activity of olive leaf extract against Candida albicans ATCC 10231 and C. dubliniensis CBS 7987 strains. Minimum inhibitory concentrations (MIC) of the extract were determined by several in vitro assays. The extract showed a concentration depended effect on the viability of C. albicans with MIC value of 46.875 mg mL-1 and C. dubliniensis with MIC value 62.5 mg mL-1. Most sensitive methods for testing the antifungal effect of the extracts were the trypan blue exclusion method and fluorescent dye exclusion method while MIC could not be determined by the method according to the EUCAST recommendation suggesting that herbal preparations contain compounds that may interfere with this susceptibility testing. The fluorescent dye exclusion method was also used for the assessment of morphological changes in the nuclei of treated cells. According to the obtained results, olive leaf extract is less effective against the tested strains than hydroxytyrosol, an olive plant constituent tested in our previous study.</abstract>

Olive leaf extract is characterized by a high content of polyphenols (oleuropein, hydroxytyrosol and their derivatives), which is associated with its therapeutic properties. The objective of the present research was to evaluate the antifungal activity of olive leaf extract against Candida albicans ATCC 10231 and C. dubliniensis CBS 7987 strains. Minimum inhibitory concentrations (MIC) of the extract were determined by several in vitro assays. The extract showed a concentration depended effect on the viability of C. albicans with MIC value of 46.875 mg mL-1 and C. dubliniensis with MIC value 62.5 mg mL-1. Most sensitive methods for testing the antifungal effect of the extracts were the trypan blue exclusion method and fluorescent dye exclusion method while MIC could not be determined by the method according to the EUCAST recommendation suggesting that herbal preparations contain compounds that may interfere with this susceptibility testing. The fluorescent dye exclusion method was also used for the assessment of morphological changes in the nuclei of treated cells. According to the obtained results, olive leaf extract is less effective against the tested strains than hydroxytyrosol, an olive plant constituent tested in our previous study.

Olive leaf extract activity against Candida albicans and C. dubliniensis – the in vitro viability study

Agency for Medicinal Products and Medical Devices of Croatia (HALMED) 10 000 Zagreb, Croatia

Institute for Medical Research and Occupational Health 10 000 Zagreb, Croatia

University of Zagreb Faculty of Food Technology and Biotechnology 10 000 Zagreb, Croatia

University of Zagreb Faculty of Science Division of Biology 10 000 Zagreb, Croatia

Acta Pharmaceutica

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 License.

Olive leaf extract is characterized by a high content of polyphenols (oleuropein, hydroxytyrosol and their derivatives), which is associated with its...

Olive leaf extract activity against Candida albicans and C. dubliniensis – the in vitro viability study

Published Online: Jun 29, 2016

Page range: 411 - 421

Accepted: Feb 21, 2016

DOI: https://doi.org/10.1515/acph-2016-0033

Keywords
antifungal activity, Candida albicans, Candida dubliniensis, olive leaf extract

© by Nataša Zorić

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 License.

Olive leaf extract activity against Candida albicans and C. dubliniensis – the in vitro viability study

Published Online: Jun 29, 2016

Page range: 411 - 421

Accepted: Feb 21, 2016

DOI: https://doi.org/10.1515/acph-2016-0033

Keywordsantifungal activity, Candida albicans, Candida dubliniensis, olive leaf extract

© by Nataša Zorić

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 License.

Keywords
antifungal activity, Candida albicans, Candida dubliniensis, olive leaf extract